Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.7.3 extracted from

  • Gehring, U.; Arnon, D.I.
    Purification and properties of alpha-ketoglutarate synthase from a photosynthetic bacterium (1972), J. Biol. Chem., 247, 6963-6969.
    View publication on PubMed

General Stability

General Stability Organism
stabilized under anaerobic conditions by a combination of dimercaptoethanol and FeSO4 Prosthecochloris vibrioformis f. thiosulfatophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
succinyl-CoA pH and temperature not specified in the publication Prosthecochloris vibrioformis f. thiosulfatophilum
2.6
-
2-oxoglutarate pH and temperature not specified in the publication Prosthecochloris vibrioformis f. thiosulfatophilum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no effect of Mg2+ Prosthecochloris vibrioformis f. thiosulfatophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
220000
-
sucrose density gradient sedimentation Prosthecochloris vibrioformis f. thiosulfatophilum

Organism

Organism UniProt Comment Textmining
Prosthecochloris vibrioformis f. thiosulfatophilum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Prosthecochloris vibrioformis f. thiosulfatophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.25
-
pH and temperature not specified in the publication Prosthecochloris vibrioformis f. thiosulfatophilum

Storage Stability

Storage Stability Organism
0°C, anaerobic conditions, 2,3-dimercaptoethanol plus FeSO4 Prosthecochloris vibrioformis f. thiosulfatophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ of several ferredoxins tested, only those from the photosynthetic bacteria Chlorobium thiosulfatophilum, Chromatium, and Rhodospirillum rubrum are effective. The ferredoxins of Clostridium pasteurianum and spinach are poor substitutes for those of photosynthetic bacteria Prosthecochloris vibrioformis f. thiosulfatophilum 2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
r

Synonyms

Synonyms Comment Organism
alpha-ketoglutarate synthase
-
Prosthecochloris vibrioformis f. thiosulfatophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 7.8 optimum with a sharp drop on the acidic side Prosthecochloris vibrioformis f. thiosulfatophilum