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Literature summary for 1.2.7.3 extracted from

  • Mai, X.; Adams, M.W.W.
    Characterization of a fourth type of 2-keto acid-oxidizing enzyme from a hyperthermophilic archaeon: 2-ketoglutarate ferredoxin oxidoreductase from Thermococcus litoralis (1996), J. Bacteriol., 178, 5890-5896.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
thiamine diphosphate 0.9 mol per mol of enzyme, stimulates but is not essential, Km: 0.009 mM Thermococcus litoralis

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 0.05 mM, complete inhibition Thermococcus litoralis
additional information no inhibition: CO, no inhibition at 5 mM: sodium benzoate, NaF, sodium hypophosphite, NaN3, KCN, 2-oxohexanoate, pyruvate, 2-oxobutyrate Thermococcus litoralis
Sodium nitrite high concentrations Thermococcus litoralis
ZnCl2 0.1 mM, complete inhibition Thermococcus litoralis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
oxidized ferredoxin pH 8.4, 80°C, ferredoxin from Pyrococcus furiosus Thermococcus litoralis
0.04
-
CoA pH 8.4, 80°C Thermococcus litoralis
0.25
-
2-oxoglutarate pH 8.4, 80°C Thermococcus litoralis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Thermococcus litoralis 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron-sulfur cluster contains at least four [4Fe-4S] clusters per mol of holoenzyme Thermococcus litoralis
Mg2+ stimulates, 2.5 mM Thermococcus litoralis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10000
-
2 * 43000 + 2 * 29000 + 2 * 23000 + 2 * 10000, alpha2-beta2-gamma2-delta2, SDS-PAGE Thermococcus litoralis
23000
-
2 * 43000 + 2 * 29000 + 2 * 23000 + 2 * 10000, alpha2-beta2-gamma2-delta2, SDS-PAGE Thermococcus litoralis
29000
-
2 * 43000 + 2 * 29000 + 2 * 23000 + 2 * 10000, alpha2-beta2-gamma2-delta2, SDS-PAGE Thermococcus litoralis
43000
-
2 * 43000 + 2 * 29000 + 2 * 23000 + 2 * 10000, alpha2-beta2-gamma2-delta2, SDS-PAGE Thermococcus litoralis
210000
-
native PAGE Thermococcus litoralis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ Thermococcus litoralis enzyme plays a role in synthesis, not in energy conservation 2-oxoglutarate + CoA + oxidized ferredoxin
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ Thermococcus litoralis DSM 5473 enzyme plays a role in synthesis, not in energy conservation 2-oxoglutarate + CoA + oxidized ferredoxin
-
?

Organism

Organism UniProt Comment Textmining
Thermococcus litoralis
-
-
-
Thermococcus litoralis DSM 5473
-
-
-

Oxidation Stability

Oxidation Stability Organism
oxygen sensitive, half-life of about 5 min Thermococcus litoralis

Purification (Commentary)

Purification (Comment) Organism
-
Thermococcus litoralis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.15
-
crude extract, pH 8.4, 80°C Thermococcus litoralis
22.3
-
pH 8.4, 80°C, purified enzyme Thermococcus litoralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + CoA + 2 oxidized ferredoxin pure enzyme does not utilize other 2-keto acids, such as pyruvate, phenylglyoxylate, indolepyruvate, 2-oxobutanoate or 2-oxoisovalerate, as substrates Thermococcus litoralis succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
?
2-oxoglutarate + CoA + 2 oxidized ferredoxin pure enzyme does not utilize other 2-keto acids, such as pyruvate, phenylglyoxylate, indolepyruvate, 2-oxobutanoate or 2-oxoisovalerate, as substrates Thermococcus litoralis DSM 5473 succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ enzyme plays a role in synthesis, not in energy conservation Thermococcus litoralis 2-oxoglutarate + CoA + oxidized ferredoxin
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ enzyme plays a role in synthesis, not in energy conservation Thermococcus litoralis DSM 5473 2-oxoglutarate + CoA + oxidized ferredoxin
-
?

Subunits

Subunits Comment Organism
octamer 2 * 43000 + 2 * 29000 + 2 * 23000 + 2 * 10000, alpha2-beta2-gamma2-delta2, SDS-PAGE Thermococcus litoralis

Synonyms

Synonyms Comment Organism
CoA-dependent 2-ketoglutarate oxidoreductase
-
Thermococcus litoralis
KGOR
-
Thermococcus litoralis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
above, at pH 8.0 Thermococcus litoralis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 95 inactive below 25°C, drastical increase of activity at 70°C Thermococcus litoralis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
half-life under anaerobic conditions: about 2 days, 2 mM dithionite, 2 mM dithiothreitol, pH 8.0 Thermococcus litoralis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Thermococcus litoralis

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 50% activity at pH 7.0 and pH 9.5, inactive at pH 5.0 and pH 10.0 Thermococcus litoralis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5
-
Sodium nitrite pH 8.4, 80°C Thermococcus litoralis