Activating Compound | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | 0.9 mol per mol of enzyme, stimulates but is not essential, Km: 0.009 mM | Thermococcus litoralis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuSO4 | 0.05 mM, complete inhibition | Thermococcus litoralis | |
additional information | no inhibition: CO, no inhibition at 5 mM: sodium benzoate, NaF, sodium hypophosphite, NaN3, KCN, 2-oxohexanoate, pyruvate, 2-oxobutyrate | Thermococcus litoralis | |
Sodium nitrite | high concentrations | Thermococcus litoralis | |
ZnCl2 | 0.1 mM, complete inhibition | Thermococcus litoralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
oxidized ferredoxin | pH 8.4, 80°C, ferredoxin from Pyrococcus furiosus | Thermococcus litoralis | |
0.04 | - |
CoA | pH 8.4, 80°C | Thermococcus litoralis | |
0.25 | - |
2-oxoglutarate | pH 8.4, 80°C | Thermococcus litoralis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Thermococcus litoralis | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron-sulfur cluster | contains at least four [4Fe-4S] clusters per mol of holoenzyme | Thermococcus litoralis | |
Mg2+ | stimulates, 2.5 mM | Thermococcus litoralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
10000 | - |
2 * 43000 + 2 * 29000 + 2 * 23000 + 2 * 10000, alpha2-beta2-gamma2-delta2, SDS-PAGE | Thermococcus litoralis |
23000 | - |
2 * 43000 + 2 * 29000 + 2 * 23000 + 2 * 10000, alpha2-beta2-gamma2-delta2, SDS-PAGE | Thermococcus litoralis |
29000 | - |
2 * 43000 + 2 * 29000 + 2 * 23000 + 2 * 10000, alpha2-beta2-gamma2-delta2, SDS-PAGE | Thermococcus litoralis |
43000 | - |
2 * 43000 + 2 * 29000 + 2 * 23000 + 2 * 10000, alpha2-beta2-gamma2-delta2, SDS-PAGE | Thermococcus litoralis |
210000 | - |
native PAGE | Thermococcus litoralis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | Thermococcus litoralis | enzyme plays a role in synthesis, not in energy conservation | 2-oxoglutarate + CoA + oxidized ferredoxin | - |
? | |
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | Thermococcus litoralis DSM 5473 | enzyme plays a role in synthesis, not in energy conservation | 2-oxoglutarate + CoA + oxidized ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus litoralis | - |
- |
- |
Thermococcus litoralis DSM 5473 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
oxygen sensitive, half-life of about 5 min | Thermococcus litoralis |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus litoralis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.15 | - |
crude extract, pH 8.4, 80°C | Thermococcus litoralis |
22.3 | - |
pH 8.4, 80°C, purified enzyme | Thermococcus litoralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + CoA + 2 oxidized ferredoxin | pure enzyme does not utilize other 2-keto acids, such as pyruvate, phenylglyoxylate, indolepyruvate, 2-oxobutanoate or 2-oxoisovalerate, as substrates | Thermococcus litoralis | succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
2-oxoglutarate + CoA + 2 oxidized ferredoxin | pure enzyme does not utilize other 2-keto acids, such as pyruvate, phenylglyoxylate, indolepyruvate, 2-oxobutanoate or 2-oxoisovalerate, as substrates | Thermococcus litoralis DSM 5473 | succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | enzyme plays a role in synthesis, not in energy conservation | Thermococcus litoralis | 2-oxoglutarate + CoA + oxidized ferredoxin | - |
? | |
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | enzyme plays a role in synthesis, not in energy conservation | Thermococcus litoralis DSM 5473 | 2-oxoglutarate + CoA + oxidized ferredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 2 * 43000 + 2 * 29000 + 2 * 23000 + 2 * 10000, alpha2-beta2-gamma2-delta2, SDS-PAGE | Thermococcus litoralis |
Synonyms | Comment | Organism |
---|---|---|
CoA-dependent 2-ketoglutarate oxidoreductase | - |
Thermococcus litoralis |
KGOR | - |
Thermococcus litoralis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
above, at pH 8.0 | Thermococcus litoralis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 95 | inactive below 25°C, drastical increase of activity at 70°C | Thermococcus litoralis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life under anaerobic conditions: about 2 days, 2 mM dithionite, 2 mM dithiothreitol, pH 8.0 | Thermococcus litoralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Thermococcus litoralis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | 50% activity at pH 7.0 and pH 9.5, inactive at pH 5.0 and pH 10.0 | Thermococcus litoralis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
Sodium nitrite | pH 8.4, 80°C | Thermococcus litoralis |