General Stability | Organism |
---|---|
0.5 mM KCl 0.5 M, 10% glycerol 1 mM dithiothreitol, stabilize | Thauera aromatica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
oxidized ferredoxin | pH 7.8, 30°C | Thauera aromatica | |
0.11 | - |
2-oxoglutarate | pH 7.8, 30°C | Thauera aromatica | |
0.29 | - |
CoA | pH 7.8, 30°C | Thauera aromatica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Thauera aromatica | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron-sulfur cluster | two [4Fe-4S] clusters per ferredoxin, 8.3 mol iron per mol of enzyme, 7.2 mol acid-labile sulfide per mol of enzyme | Thauera aromatica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
2 * 66000 + 2 * 34000, alpha2-beta2, SDS-PAGE | Thauera aromatica |
66000 | - |
2 * 66000 + 2 * 34000, alpha2-beta2, SDS-PAGE | Thauera aromatica |
200000 | - |
gel filtration | Thauera aromatica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | Thauera aromatica | it is proposed that the enzyme couples the complete acetyl-CoA oxidation to aromatic ring reduction by the use of the low-potential electron shuttle ferredoxin | 2-oxoglutarate + CoA + 2 oxidized ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thauera aromatica | O87870 and Q8RJQ9 | O87870: alpha-subunit, Q8RJQ9: beta-subunit | - |
Oxidation Stability | Organism |
---|---|
aerobic conditions, 4°C, 50% loss of activity after 1 day | Thauera aromatica |
Purification (Comment) | Organism |
---|---|
- |
Thauera aromatica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | activity is increased 10fold to 50fold in Thauera aromatica cells grown under denitrifying conditions on an aromatic substrate compared to that of cells grown on nonaromatic substrates | Thauera aromatica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.8 | - |
pH 7.8, 30°C | Thauera aromatica |
Storage Stability | Organism |
---|---|
4°C, anaerobic conditions, 100% N2-atmosphere, 4 mM MgCl2, 1 mM dithiothreitol, 2 mM thiamine diphosphate, 10% glycerol, stable for at least 1 day | Thauera aromatica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + CoA + 2 oxidized ferredoxin | specific for 2-oxoglutarate | Thauera aromatica | succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | it is proposed that the enzyme couples the complete acetyl-CoA oxidation to aromatic ring reduction by the use of the low-potential electron shuttle ferredoxin | Thauera aromatica | 2-oxoglutarate + CoA + 2 oxidized ferredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | comparison of subunit structure, molecularly and phylogenetically, of several 2-ketoacid: acceptor oxidoreductases | Thauera aromatica |
tetramer | 2 * 66000 + 2 * 34000, alpha2-beta2, SDS-PAGE | Thauera aromatica |
Synonyms | Comment | Organism |
---|---|---|
2-oxoglutarate:ferredoxin oxidoreductase | - |
Thauera aromatica |
KGOR | - |
Thauera aromatica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
2-oxoglutarate | pH 7.8, 30°C | Thauera aromatica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
glycylglycine buffer | Thauera aromatica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | comparison of cofactor binding motifs of 2-ketoacid:acceptor oxidoreductases | Thauera aromatica | |
thiamine diphosphate | 1.6 mol per mol of enzyme | Thauera aromatica |
Organism | Comment | Expression |
---|---|---|
Thauera aromatica | inducible by growth on aromatic substrates and under denitrifiying conditions 10 to 50fold | up |