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Literature summary for 1.2.7.3 extracted from

  • Dörner, E.; Boll, M.
    Properties of 2-oxoglutarate:ferredoxin oxidoreductase from Thauera aromatica and its role in enzymatic reduction of the aromatic ring (2002), J. Bacteriol., 184, 3975-3983.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
0.5 mM KCl 0.5 M, 10% glycerol 1 mM dithiothreitol, stabilize Thauera aromatica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
oxidized ferredoxin pH 7.8, 30°C Thauera aromatica
0.11
-
2-oxoglutarate pH 7.8, 30°C Thauera aromatica
0.29
-
CoA pH 7.8, 30°C Thauera aromatica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Thauera aromatica 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron-sulfur cluster two [4Fe-4S] clusters per ferredoxin, 8.3 mol iron per mol of enzyme, 7.2 mol acid-labile sulfide per mol of enzyme Thauera aromatica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
2 * 66000 + 2 * 34000, alpha2-beta2, SDS-PAGE Thauera aromatica
66000
-
2 * 66000 + 2 * 34000, alpha2-beta2, SDS-PAGE Thauera aromatica
200000
-
gel filtration Thauera aromatica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ Thauera aromatica it is proposed that the enzyme couples the complete acetyl-CoA oxidation to aromatic ring reduction by the use of the low-potential electron shuttle ferredoxin 2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
?

Organism

Organism UniProt Comment Textmining
Thauera aromatica O87870 and Q8RJQ9 O87870: alpha-subunit, Q8RJQ9: beta-subunit
-

Oxidation Stability

Oxidation Stability Organism
aerobic conditions, 4°C, 50% loss of activity after 1 day Thauera aromatica

Purification (Commentary)

Purification (Comment) Organism
-
Thauera aromatica

Source Tissue

Source Tissue Comment Organism Textmining
additional information activity is increased 10fold to 50fold in Thauera aromatica cells grown under denitrifying conditions on an aromatic substrate compared to that of cells grown on nonaromatic substrates Thauera aromatica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.8
-
pH 7.8, 30°C Thauera aromatica

Storage Stability

Storage Stability Organism
4°C, anaerobic conditions, 100% N2-atmosphere, 4 mM MgCl2, 1 mM dithiothreitol, 2 mM thiamine diphosphate, 10% glycerol, stable for at least 1 day Thauera aromatica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + CoA + 2 oxidized ferredoxin specific for 2-oxoglutarate Thauera aromatica succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ it is proposed that the enzyme couples the complete acetyl-CoA oxidation to aromatic ring reduction by the use of the low-potential electron shuttle ferredoxin Thauera aromatica 2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
?

Subunits

Subunits Comment Organism
More comparison of subunit structure, molecularly and phylogenetically, of several 2-ketoacid: acceptor oxidoreductases Thauera aromatica
tetramer 2 * 66000 + 2 * 34000, alpha2-beta2, SDS-PAGE Thauera aromatica

Synonyms

Synonyms Comment Organism
2-oxoglutarate:ferredoxin oxidoreductase
-
Thauera aromatica
KGOR
-
Thauera aromatica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17
-
2-oxoglutarate pH 7.8, 30°C Thauera aromatica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
glycylglycine buffer Thauera aromatica

Cofactor

Cofactor Comment Organism Structure
additional information comparison of cofactor binding motifs of 2-ketoacid:acceptor oxidoreductases Thauera aromatica
thiamine diphosphate 1.6 mol per mol of enzyme Thauera aromatica

Expression

Organism Comment Expression
Thauera aromatica inducible by growth on aromatic substrates and under denitrifiying conditions 10 to 50fold up