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Literature summary for 1.2.7.3 extracted from

  • Yamamoto, M.; Arai, H.; Ishii, M.; Igarashi, Y.
    Characterization of two different 2-oxoglutarate:ferredoxin oxidoreductases from Hydrogenobacter thermophilus TK-6 (2003), Biochem. Biophys. Res. Commun., 312, 1297-1302.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of forDABGE in Escherichia coli JM109, structure of gene cluster Hydrogenobacter thermophilus
expression of korAB in Escherichia coli JM109, structure of gene cluster Hydrogenobacter thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
CoA pH 7.8, 70┬░C, recombinant For Hydrogenobacter thermophilus
0.08
-
CoA pH 7.8, 70┬░C, recombinant Kor Hydrogenobacter thermophilus
1.4
-
2-oxoglutarate pH 7.8, 70┬░C, recombinant Kor Hydrogenobacter thermophilus
2.9
-
2-oxoglutarate pH 7.8, 70┬░C, recombinant For Hydrogenobacter thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
230000
-
recombinant For, gel filtration Hydrogenobacter thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxoglutarate + CoA + oxidized ferredoxin Hydrogenobacter thermophilus one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen succinyl-CoA + CO2 + reduced ferredoxin
-
?
2-oxoglutarate + CoA + oxidized ferredoxin Hydrogenobacter thermophilus TK-6 / IAM 12695 one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen succinyl-CoA + CO2 + reduced ferredoxin
-
?

Organism

Organism UniProt Comment Textmining
Hydrogenobacter thermophilus D3DIA1 and D3DIA2 and D3DIA3 and D3DIA0 and Q93RA0 D3DIA1: subunit forA, D3DIA2: subunit forB, D3DIA3: subunit forG, D3DIA0: subunit forD, Q93RA0: subunit forE; two distinct OGOR enzymes termed Kor and For
-
Hydrogenobacter thermophilus Q9AJM0 and Q9AJL9 Q9AJM0: subunit korA, Q9AJL9: subunit korB; two distinct OGOR enzymes termed Kor and For
-
Hydrogenobacter thermophilus TK-6 / IAM 12695 D3DIA1 and D3DIA2 and D3DIA3 and D3DIA0 and Q93RA0 D3DIA1: subunit forA, D3DIA2: subunit forB, D3DIA3: subunit forG, D3DIA0: subunit forD, Q93RA0: subunit forE; two distinct OGOR enzymes termed Kor and For
-
Hydrogenobacter thermophilus TK-6 / IAM 12695 Q9AJM0 and Q9AJL9 Q9AJM0: subunit korA, Q9AJL9: subunit korB; two distinct OGOR enzymes termed Kor and For
-

Purification (Commentary)

Purification (Comment) Organism
-
Hydrogenobacter thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
additional information
-
the specific activity of For is approximately one-tenth of that of Kor Hydrogenobacter thermophilus
35.2
-
pH 7.8, 70┬░C, recombinant Kor Hydrogenobacter thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + CoA + oxidized ferredoxin
-
Hydrogenobacter thermophilus succinyl-CoA + CO2 + reduced ferredoxin
-
?
2-oxoglutarate + CoA + oxidized ferredoxin one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen Hydrogenobacter thermophilus succinyl-CoA + CO2 + reduced ferredoxin
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
-
Hydrogenobacter thermophilus TK-6 / IAM 12695 succinyl-CoA + CO2 + reduced ferredoxin
-
?
2-oxoglutarate + CoA + oxidized ferredoxin one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen Hydrogenobacter thermophilus TK-6 / IAM 12695 succinyl-CoA + CO2 + reduced ferredoxin
-
?

Subunits

Subunits Comment Organism
oligomer the For enzyme has a five-subunit-type structure, the molar ratio of ForA:ForB:ForG:ForD:ForE is 1.0:1.04:1.02:0.98:0.2, the sum of the calculated MWs of the five For subunits is 136 kDa Hydrogenobacter thermophilus

Synonyms

Synonyms Comment Organism
FOR enzyme
-
Hydrogenobacter thermophilus
KOR enzyme
-
Hydrogenobacter thermophilus
OGOR
-
Hydrogenobacter thermophilus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
80
-
recombinant For enzyme Hydrogenobacter thermophilus

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
60 90 60┬░C: about 35% of maximal activity, 90┬░C: about 50% of maximal activity, recombinant For, the recombinant For enzyme is inactive at room temperature Hydrogenobacter thermophilus

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
70
-
recombinant Kor, about 70% loss of activity under aerobic conditions, no loss of activity under anaerobic conditions Hydrogenobacter thermophilus
80
-
recombinant For, about 70% loss of activity under aerobic conditions, no loss of activity under anaerobic conditions Hydrogenobacter thermophilus
80
-
recombinant Kor, about 95% loss of activity under aerobic conditions, about 25% loss of activity under anaerobic conditions Hydrogenobacter thermophilus
90
-
recombinant For, about 90% loss of activity under aerobic conditions, about 30% loss of activity under anaerobic conditions Hydrogenobacter thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
recombinant Kor Hydrogenobacter thermophilus
8.7
-
recombinant For, at 70┬░C, optimum varies between buffers Hydrogenobacter thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
7 9.7 pH 7.0: about 45% of maximal activity, pH 9.7: about 65% of maximal activity, recombinant For Hydrogenobacter thermophilus

Expression

Organism Comment Expression
Hydrogenobacter thermophilus the For enzyme is expressed constitutively during oxygen respiration. Little expression of For is observed during cultivation under conditions of nitrate respiration additional information
Hydrogenobacter thermophilus the Kor enzyme is constitutively expressed during respiration of either oxygen or nitrate respiration additional information

General Information

General Information Comment Organism
physiological function the For enzyme supports the optimal growth of the organism in the presence of oxygen Hydrogenobacter thermophilus