General Stability | Organism |
---|---|
98% loss of activity after 15 min in an aerobic buffer | Moorella thermoacetica |
the presence of CO has no apparent effect on the stability | Moorella thermoacetica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | 0.04 mM, 60% inhibition within 10 s, CO reverses inhibition | Moorella thermoacetica | |
KCN | CO reverses cyanide inhibition, but promotes reaction with methyl iodide | Moorella thermoacetica | |
methyl iodide | CO promotes inhibition; irreversible inhibition | Moorella thermoacetica | |
additional information | pyruvate and ATP have no effect | Moorella thermoacetica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.03 | - |
methyl viologen | 50°C, pH 8.4 | Moorella thermoacetica | |
3.03 | - |
methyl viologen | pH 8.4, 50°C | Moorella thermoacetica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Moorella thermoacetica | |
Iron | contains 11 mol of iron and 14 mol of acid-labile sulfur per mol of alphabeta dimer | Moorella thermoacetica | |
Ni2+ | - |
Moorella thermoacetica | |
Nickel | contains 2 mol of nickel per mol of alphabeta dimer | Moorella thermoacetica | |
Zinc | contains 1 mol of zinc per mol of alphabeta dimer | Moorella thermoacetica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
3 * 71000, beta, + 3 * 78000, alpha, SDS-PAGE | Moorella thermoacetica |
71000 | - |
3 * 78000 + 3 * 71000, alpha3,beta3, SDS-PAGE | Moorella thermoacetica |
78000 | - |
3 * 71000, beta, + 3 * 78000, alpha, SDS-PAGE | Moorella thermoacetica |
78000 | - |
3 * 78000 + 3 * 71000, alpha3,beta3, SDS-PAGE | Moorella thermoacetica |
155000 | - |
sedimentation equilibrium centrifugation | Moorella thermoacetica |
161000 | - |
pore limit gel electrophoresis | Moorella thermoacetica |
436000 | - |
gel filtration | Moorella thermoacetica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO + H2O + oxidized ferredoxin | Moorella thermoacetica | initial step of CO metabolism in acetogenic bacteria | CO2 + reduced ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moorella thermoacetica | - |
- |
- |
Moorella thermoacetica | - |
formerly Clostridium thermoaceticum | - |
Moorella thermoacetica | - |
ATCC 39073 | - |
Oxidation Stability | Organism |
---|---|
addition of the enzyme to an aerobic buffer, 50 mM Tris/HCl, pH 7.6 results in a 98% loss of activity within 15 min, presence of CO has no apparent effect on the stability of the enzyme | Moorella thermoacetica |
extreme oxygen lability | Moorella thermoacetica |
Purification (Comment) | Organism |
---|---|
- |
Moorella thermoacetica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
675 | - |
electron acceptor methyl viologen | Moorella thermoacetica |
Storage Stability | Organism |
---|---|
-20°C or 5°C, 50 mM Tris/HCl, pH 7.5, 2 mM sodium dithionite, 0.2 mM methyl viologen, 50% v/v glycerol, stable for more than 1 month | Moorella thermoacetica |
-20°C, stored frozen in an oxygen-free atmosphere in buffer containing glycerol and 2 mM dithionite, not stable for more than 1 month | Moorella thermoacetica |
10°C, Tris/HCl, pH 7.6, enzyme is active for at least 2 days | Moorella thermoacetica |
5°C, stored in an oxygen-free atmosphere in buffer containing glycerol and 2 mM dithionite, not stable for more than 1 month | Moorella thermoacetica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO + H2O + ferredoxin | - |
Moorella thermoacetica | CO2 + reduced ferredoxin | - |
? | |
CO + H2O + flavodoxin | - |
Moorella thermoacetica | CO2 + reduced flavodoxin | - |
? | |
CO + H2O + FMN | - |
Moorella thermoacetica | CO2 + FMNH2 | - |
? | |
CO + H2O + methyl viologen | - |
Moorella thermoacetica | CO2 + reduced methyl viologen | - |
? | |
CO + H2O + methylene blue | - |
Moorella thermoacetica | CO2 + reduced methylene blue | - |
? | |
CO + H2O + oxidized ferredoxin | ferredoxin I and II | Moorella thermoacetica | CO2 + reduced ferredoxin | - |
? | |
CO + H2O + oxidized ferredoxin | initial step of CO metabolism in acetogenic bacteria | Moorella thermoacetica | CO2 + reduced ferredoxin | - |
? | |
CO + H2O + oxidized flavodoxin | - |
Moorella thermoacetica | CO2 + reduced flavodoxin | - |
? | |
CO + H2O + oxidized methyl viologen | - |
Moorella thermoacetica | CO2 + reduced methyl viologen | - |
? | |
CO + H2O + oxidized methylene blue | - |
Moorella thermoacetica | CO2 + reduced methylene blue | - |
? | |
CO + H2O + oxidized rubredoxin | most efficient electron acceptor | Moorella thermoacetica | CO2 + reduced rubredoxin | - |
? | |
CO + H2O + oxidized viologen | - |
Moorella thermoacetica | CO2 + reduced viologen | - |
? | |
CO + H2O + rubredoxin | most efficient electron acceptor | Moorella thermoacetica | CO2 + reduced rubredoxin | - |
? | |
additional information | pure enzyme has no hydrogenase or formate dehydrogenase activity | Moorella thermoacetica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 3 * 71000, beta, + 3 * 78000, alpha, SDS-PAGE | Moorella thermoacetica |
hexamer | 3 * 78000 + 3 * 71000, alpha3,beta3, SDS-PAGE | Moorella thermoacetica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 70 | purification can be done at 25°C without loss of activity, heating for 30 min at 67°C leads to apparent loss, heating for only a few min at 70°C activates the enzyme | Moorella thermoacetica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 8.4 | - |
Moorella thermoacetica |