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Literature summary for 1.2.7.4 extracted from

  • Seravalli, J.; Ragsdale, S.W.
    13C NMR characterization of an exchange reaction between CO and CO2 catalyzed by carbon monoxide dehydrogenase (2008), Biochemistry, 47, 6770-6781.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Carboxydothermus hydrogenoformans

Protein Variants

Protein Variants Comment Organism
C56A devoid of CO oxidation activity Carboxydothermus hydrogenoformans
K563L very low CO oxidation activity Carboxydothermus hydrogenoformans

Inhibitors

Inhibitors Comment Organism Structure
cyanide inhibitory at 5 mM; strong competitive inhibitor Carboxydothermus hydrogenoformans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
CO at 70°C Carboxydothermus hydrogenoformans
4
-
methyl viologen at 70°C Carboxydothermus hydrogenoformans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ contains 9 Fe+ per monomer Carboxydothermus hydrogenoformans
Ni2+ contains 0.1 Ni2+ per monomer Carboxydothermus hydrogenoformans

Organism

Organism UniProt Comment Textmining
Carboxydothermus hydrogenoformans
-
strain DSMZ 6008
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Carboxydothermus hydrogenoformans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO + H2O + acceptor
-
Carboxydothermus hydrogenoformans CO2 + reduced acceptor
-
?
CO + H2O + methyl viologen
-
Carboxydothermus hydrogenoformans CO2 + reduced methyl viologen
-
r

Synonyms

Synonyms Comment Organism
CODH-II
-
Carboxydothermus hydrogenoformans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1500
-
CO at 20°C Carboxydothermus hydrogenoformans
15900
-
CO at 70°C Carboxydothermus hydrogenoformans