Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Carboxydothermus hydrogenoformans |
Protein Variants | Comment | Organism |
---|---|---|
C56A | devoid of CO oxidation activity | Carboxydothermus hydrogenoformans |
K563L | very low CO oxidation activity | Carboxydothermus hydrogenoformans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | inhibitory at 5 mM; strong competitive inhibitor | Carboxydothermus hydrogenoformans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
CO | at 70°C | Carboxydothermus hydrogenoformans | |
4 | - |
methyl viologen | at 70°C | Carboxydothermus hydrogenoformans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | contains 9 Fe+ per monomer | Carboxydothermus hydrogenoformans | |
Ni2+ | contains 0.1 Ni2+ per monomer | Carboxydothermus hydrogenoformans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carboxydothermus hydrogenoformans | - |
strain DSMZ 6008 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Carboxydothermus hydrogenoformans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO + H2O + acceptor | - |
Carboxydothermus hydrogenoformans | CO2 + reduced acceptor | - |
? | |
CO + H2O + methyl viologen | - |
Carboxydothermus hydrogenoformans | CO2 + reduced methyl viologen | - |
r |
Synonyms | Comment | Organism |
---|---|---|
CODH-II | - |
Carboxydothermus hydrogenoformans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1500 | - |
CO | at 20°C | Carboxydothermus hydrogenoformans | |
15900 | - |
CO | at 70°C | Carboxydothermus hydrogenoformans |