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Literature summary for 1.2.7.4 extracted from

  • Ha, S.W.; Korbas, M.; Klepsch, M.; Meyer-Klaucke, W.; Meyer, O.; Svetlitchnyi, V.
    Interaction of potassium cyanide with the [Ni-4Fe-5S] active site cluster of CO dehydrogenase from Carboxydothermus hydrogenoformans (2007), J. Biol. Chem., 282, 10639-10646.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
cyanide 0.075 mM, reversible, competitive inhibition of reduced CODHII with respect to the substrate carbon monoxide, which protects reduced CODHII against inhibition by cyanide, inhibited CODHII regains initial activity after a 15-25-min incubation at 70°C with dithionite or Ti(III) citrate under CO or N2, while slower and partial reactivation to 30-50% of the initial activity occurs with dithiothreitol or without reductants Carboxydothermus hydrogenoformans
potassium cyanide competitive inhibitor of reduced CODHII with respect to the substrate CO, inhibition of dithionite- or Ti(III) citrate-reduced CODHII by potassium cyanide is fully reversible since the enzyme can be completely reactivated, sodium sulfide has no effect on the reactivation of cyanide-inhibited CODHII in the presence of dithionite Carboxydothermus hydrogenoformans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ contains one Ni-4Fe-5S cluster Carboxydothermus hydrogenoformans
Ni2+ contains one Ni-4Fe-5S cluster Carboxydothermus hydrogenoformans

Organism

Organism UniProt Comment Textmining
Carboxydothermus hydrogenoformans
-
strain Z-2901 (DSM 6008)
-
Carboxydothermus hydrogenoformans Z-2901 / DSM 6008
-
strain Z-2901 (DSM 6008)
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO + H2O + methyl viologen
-
Carboxydothermus hydrogenoformans CO2 + reduced methyl viologen
-
?
CO + H2O + methyl viologen
-
Carboxydothermus hydrogenoformans Z-2901 / DSM 6008 CO2 + reduced methyl viologen
-
?

Synonyms

Synonyms Comment Organism
CO dehydrogenase
-
Carboxydothermus hydrogenoformans
CODHII
-
Carboxydothermus hydrogenoformans
Ni-Fe carbon monoxide dehydrogenase II
-
Carboxydothermus hydrogenoformans

Cofactor

Cofactor Comment Organism Structure
Ni-4Fe-5S center active site cluster Carboxydothermus hydrogenoformans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0217
-
cyanide at 23°C, in 4 mM dithionite, and 4 mM dithiothreitol Carboxydothermus hydrogenoformans