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Literature summary for 1.2.7.4 extracted from

  • Nishimura, H.; Nomura, Y.; Iwata, E.; Sato, N.; Sako, Y.
    Purification and characterization of carbon monoxide dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix (2010), Fish. Sci., 76, 999-1006.
No PubMed abstract available

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12600
-
1 * 86600 + 2 * 34500 + 1 * 12600, SDS-PAGE Aeropyrum pernix
34500
-
1 * 86600 + 2 * 34500 + 1 * 12600, SDS-PAGE Aeropyrum pernix
86600
-
1 * 86600 + 2 * 34500 + 1 * 12600, SDS-PAGE Aeropyrum pernix
163700
-
calculated from amino acid sequence Aeropyrum pernix
210000
-
gel filtration Aeropyrum pernix

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix
-
-
-
Aeropyrum pernix TB5
-
-
-

Oxidation Stability

Oxidation Stability Organism
100% of activity remains after 48 h of oxygen exposure, and after 168 h of air exposure, activity is 88% of the initial activity Aeropyrum pernix

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, CHT-I column chromatography, and Superdex 200 gel filtration Aeropyrum pernix

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.96
-
after 12.92fold purification, at 95°C, pH 8.0, using methylene blue as electron acceptor Aeropyrum pernix
2.08
-
after 12.92fold purification, at 95°C, pH 8.0, using methyl viologen as electron acceptor Aeropyrum pernix
2.45
-
after 12.92fold purification, at 95°C, pH 8.0, using NADP+ as electron acceptor Aeropyrum pernix
2.47
-
after 12.92fold purification, at 95°C, pH 8.0, using NAD+ as electron acceptor Aeropyrum pernix

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO + H2O + methyl viologen
-
Aeropyrum pernix CO2 + reduced methyl viologen + H+
-
?
CO + H2O + methyl viologen
-
Aeropyrum pernix TB5 CO2 + reduced methyl viologen + H+
-
?
CO + H2O + NAD+ the specific activity is increased by approximately 20% with NAD+ as the electron acceptor compared to methyl viologen Aeropyrum pernix CO2 + NADH + H+
-
?
CO + H2O + NAD+ the specific activity is increased by approximately 20% with NAD+ as the electron acceptor compared to methyl viologen Aeropyrum pernix TB5 CO2 + NADH + H+
-
?
CO + H2O + NADP+ the specific activity is increased by approximately 20% with NADP+ as the electron acceptor compared to methyl viologen Aeropyrum pernix CO2 + NADPH + H+
-
?
CO + H2O + NADP+ the specific activity is increased by approximately 20% with NADP+ as the electron acceptor compared to methyl viologen Aeropyrum pernix TB5 CO2 + NADPH + H+
-
?

Subunits

Subunits Comment Organism
heterotetramer 1 * 86600 + 2 * 34500 + 1 * 12600, SDS-PAGE Aeropyrum pernix

Synonyms

Synonyms Comment Organism
CODH
-
Aeropyrum pernix

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
Aeropyrum pernix

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
75 100 about 65% activity at 75°C, about 75% activity at 80°C, about 80% activity at 85°C, about 90% activity at 90°C, 100% activity at 95°C, about 30% activity at 100°C, respectively Aeropyrum pernix