Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12600 | - |
1 * 86600 + 2 * 34500 + 1 * 12600, SDS-PAGE | Aeropyrum pernix |
34500 | - |
1 * 86600 + 2 * 34500 + 1 * 12600, SDS-PAGE | Aeropyrum pernix |
86600 | - |
1 * 86600 + 2 * 34500 + 1 * 12600, SDS-PAGE | Aeropyrum pernix |
163700 | - |
calculated from amino acid sequence | Aeropyrum pernix |
210000 | - |
gel filtration | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | - |
- |
- |
Aeropyrum pernix TB5 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
100% of activity remains after 48 h of oxygen exposure, and after 168 h of air exposure, activity is 88% of the initial activity | Aeropyrum pernix |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, CHT-I column chromatography, and Superdex 200 gel filtration | Aeropyrum pernix |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.96 | - |
after 12.92fold purification, at 95°C, pH 8.0, using methylene blue as electron acceptor | Aeropyrum pernix |
2.08 | - |
after 12.92fold purification, at 95°C, pH 8.0, using methyl viologen as electron acceptor | Aeropyrum pernix |
2.45 | - |
after 12.92fold purification, at 95°C, pH 8.0, using NADP+ as electron acceptor | Aeropyrum pernix |
2.47 | - |
after 12.92fold purification, at 95°C, pH 8.0, using NAD+ as electron acceptor | Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO + H2O + methyl viologen | - |
Aeropyrum pernix | CO2 + reduced methyl viologen + H+ | - |
? | |
CO + H2O + methyl viologen | - |
Aeropyrum pernix TB5 | CO2 + reduced methyl viologen + H+ | - |
? | |
CO + H2O + NAD+ | the specific activity is increased by approximately 20% with NAD+ as the electron acceptor compared to methyl viologen | Aeropyrum pernix | CO2 + NADH + H+ | - |
? | |
CO + H2O + NAD+ | the specific activity is increased by approximately 20% with NAD+ as the electron acceptor compared to methyl viologen | Aeropyrum pernix TB5 | CO2 + NADH + H+ | - |
? | |
CO + H2O + NADP+ | the specific activity is increased by approximately 20% with NADP+ as the electron acceptor compared to methyl viologen | Aeropyrum pernix | CO2 + NADPH + H+ | - |
? | |
CO + H2O + NADP+ | the specific activity is increased by approximately 20% with NADP+ as the electron acceptor compared to methyl viologen | Aeropyrum pernix TB5 | CO2 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 1 * 86600 + 2 * 34500 + 1 * 12600, SDS-PAGE | Aeropyrum pernix |
Synonyms | Comment | Organism |
---|---|---|
CODH | - |
Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Aeropyrum pernix |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | 100 | about 65% activity at 75°C, about 75% activity at 80°C, about 80% activity at 85°C, about 90% activity at 90°C, 100% activity at 95°C, about 30% activity at 100°C, respectively | Aeropyrum pernix |