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Literature summary for 1.2.7.6 extracted from

  • Park, M.O.; Mizutani, T.; Jones, P.R.
    Glyceraldehyde-3-phosphate ferredoxin oxidoreductase from Methanococcus maripaludis (2007), J. Bacteriol., 189, 7281-7289.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.7.6

Activating Compound

Activating Compound Comment Organism Structure
isopropyl-beta-D-thiogalactopyranoside 1 mM induces cultures at the exponential phase of growth Methanococcus maripaludis
L-cysteine reversibly activates GAPOR purified from M9/Mo+ cultures Methanococcus maripaludis
Sodium molybdate
-
 Methanococcus maripaludis

Application

Application Comment Organism
degradation the major physiological role of GAPOR in Methanococcus maripaludis most likely involves only nonoptimal growth conditions Methanococcus maripaludis

Cloned(Commentary)

Cloned (Comment) Organism
pET46-Ek/LIC-GAPOR vector expressed in Escherichia coli strains BL21(DE3) or Rosetta-gami 2(DE3) Methanococcus maripaludis

Inhibitors

Inhibitors Comment Organism Structure
ATP 0.001-0.01 mM completely inhibits Methanococcus maripaludis
D-Glyceraldehyde-3-phosphate recombinant GAPOR is posttranscriptionally regulated as it exhibits pronounced and irreversible substrate inhibition. Inhibition is independent of benzyl viologen Methanococcus maripaludis
additional information addition of 0.001 mM AMP, NADH, or NADPH has no effect on GAPOR activity Methanococcus maripaludis
O2 reversibly inactivates GAPOR purified from M9/Mo+ cultures. GAPOR activity of crude lysates is irreversibly inactivated Methanococcus maripaludis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Methanococcus maripaludis
S2- Mo-GAPOR expressed in Escherichia coli strain BL21(DE3) is estimated to contain 6.1 g-atoms of acid-labile sulfide per mol of protein Methanococcus maripaludis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 gel filtration Methanococcus maripaludis

Organism

Organism UniProt Comment Textmining
Methanococcus maripaludis A4FXX8
-
-

Purification (Commentary)

Purification (Comment) Organism
to more than 75% homogeneity, by gel filtration Methanococcus maripaludis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8
-
 maximum specific activity of protein for Mo-GAPOR expressed in Escherichia coli strain BL21(DE3) Methanococcus maripaludis
120
-
 Mo-GAPOR purified from Escherichia coli strain Rosetta-gami 2(DE3) Methanococcus maripaludis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde-3-phosphate + H2O + oxidized benzyl viologen only GAPOR catalyzes D-glyceraldehyde-3-phosphate oxidation, catalysis under autotrophic conditions Methanococcus maripaludis 3-phospho-D-glycerate + H+ + reduced benzyl viologen
-
 ir
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin
-
 Methanococcus maripaludis 3-phospho-D-glycerate + H+ + reduced ferredoxin
-
 ?

Subunits

Subunits Comment Organism
monomer gel filtration Methanococcus maripaludis

Synonyms

Synonyms Comment Organism
GAPOR
-
 Methanococcus maripaludis
glyceraldehyde-3-phosphate ferredoxin oxidoreductase
-
 Methanococcus maripaludis

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
 Methanococcus maripaludis
molybdopterin content of Mo-GAPOR expressed in Escherichia coli strain BL21(DE3) is estimated to be 0.35 g-atoms per mol of protein Methanococcus maripaludis
additional information GAPOR obtained from cells grown in medium containing tungsten and molybdenum or in medium without added tungsten and molybdenum do not display any activity Methanococcus maripaludis