Literature summary for 1.2.7.6 extracted from

Scott, I.M.; Rubinstein, G.M.; Poole, F.L.; Lipscomb, G.L.; Schut, G.J.; Williams-Rhaesa, A.M.; Stevenson, D.M.; Amador-Noguez, D.; Kelly, R.M.; Adams, M.W.W.
The thermophilic biomass-degrading bacterium Caldicellulosiruptor bescii utilizes two enzymes to oxidize glyceraldehyde 3-phosphate during glycolysis (2019), J. Biol. Chem., 294, 9995-10005 .

Search for more literature for 1.2.7.6

Inhibitors

Inhibitors	Comment	Organism	Structure
dihydroxyacetone phosphate	10 mM, 70% inhibition	Caldicellulosiruptor bescii
additional information	bifunctional phosphoglycerate kinase/triose-phosphate isomerase is a strong inhibitor of GOR	Caldicellulosiruptor bescii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0389	-	oxidized ferredoxin	pH 8.0, 70°C	Caldicellulosiruptor bescii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Tungsten	large subunit GOR-L contains a single tungstopterin site and one iron-sulfur [4Fe-4S] cluster	Caldicellulosiruptor bescii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
73000	-	gel filtration	Caldicellulosiruptor bescii
315000	-	gel filtration	Caldicellulosiruptor bescii

Organism

Organism	UniProt	Comment	Textmining
Caldicellulosiruptor bescii	B9MQI2 and B9MQI1	B9MQI2 i.e. large subunit GOR-L, B9MQI1 i.e. 4Fe-4S ferredoxin iron-sulfur binding domain protein, GOR-S	-
Caldicellulosiruptor bescii DSM 6725	B9MQI2 and B9MQI1	B9MQI2 i.e. large subunit GOR-L, B9MQI1 i.e. 4Fe-4S ferredoxin iron-sulfur binding domain protein, GOR-S	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
48.3	-	substrate glyceraldehyde 3-phosphate, pH 8.0, 70°C	Caldicellulosiruptor bescii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glyceraldehyde + H2O + oxidized ferredoxin	-	Caldicellulosiruptor bescii	glycerate + H+ + reduced ferredoxin	-	?
glyceraldehyde + H2O + oxidized ferredoxin	-	Caldicellulosiruptor bescii DSM 6725	glycerate + H+ + reduced ferredoxin	-	?
glyceraldehyde 3-phosphate + H2O + oxidized ferredoxin	-	Caldicellulosiruptor bescii	glycerate 3-phosphate + H+ + reduced ferredoxin	-	?
glyceraldehyde 3-phosphate + H2O + oxidized ferredoxin	-	Caldicellulosiruptor bescii DSM 6725	glycerate 3-phosphate + H+ + reduced ferredoxin	-	?

Subunits

Subunits	Comment	Organism
dimer	1 * 60000, large subunit, 1 * 15000, small subunit, and tetramer of homodimers,	Caldicellulosiruptor bescii
octamer	tetramer of homodimers, 4 * 60000, large subunit, 4 * 15000, small subunit, and dimer	Caldicellulosiruptor bescii

Synonyms

Synonyms	Comment	Organism
Athe_0820	gene name, 4Fe-4S ferredoxin iron-sulfur binding domain	Caldicellulosiruptor bescii
Athe_0821	-	Caldicellulosiruptor bescii
glyceraldehyde 3-phosphate oxidoreductase	-	Caldicellulosiruptor bescii
Gor	-	Caldicellulosiruptor bescii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
5.57	-	oxidized ferredoxin	pH 8.0, 70°C	Caldicellulosiruptor bescii

Cofactor

Cofactor	Comment	Organism	Structure
[4Fe-4S]-center	large subunit GOR-L contains one iron-sulfur [4Fe-4S] cluster, small subunit GOR-S contains four [4Fe-4S] cluster	Caldicellulosiruptor bescii

General Information

General Information	Comment	Organism
physiological function	deletion of either subunit results in a distinct growth phenotype on both C5 and C6 sugars, with an increased lag phase, but higher cell densities. GOR functions in parallel with the conventional glyceraldehyde-3-phosphate dehydrogenase	Caldicellulosiruptor bescii

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
47	-	glyceraldehyde 3-phosphate	pH 8, 70°C	Caldicellulosiruptor bescii

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)