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Literature summary for 1.2.7.B3 extracted from

  • Bevers, L.E.; Bol, E.; Hagedoorn, P.L.; Hagen, W.R.
    WOR5, a novel tungsten-containing aldehyde oxidoreductase from Pyrococcus furiosus with a broad substrate specificity (2005), J. Bacteriol., 187, 7056-7061.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.7.B3

Metals/Ions

Metals/Ions Comment Organism Structure
Iron contains one [4Fe-4S] cluster per subunit Pyrococcus furiosus
Tungsten one tungstobispterin cofactor per subunit Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 2 * 67000, SDS-PAGE Pyrococcus furiosus
135000
-
 non-denaturing PAGE Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pyrococcus furiosus ferredoxin can be the physiological electron acceptor ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus E7FHH1 and Q8U0V2 E7FHH1: alpha-subunit, Q8U0V2: electron-transfer beta-subunit
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.6
-
 pH 8.4, 60°C Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-ethylhexanal + H2O + oxidized benzyl viologen Vmax/Km is 56% compared to the value for hexanal Pyrococcus furiosus 2-ethylhexanoate + reduced benzyl viologen + H+
-
 ?
2-methylbutyraldehyde + H2O + oxidized benzyl viologen Vmax/Km is 20% compared to the value for hexanal Pyrococcus furiosus 2-methylbutyrate + reduced benzyl viologen + H+
-
 ?
2-methylvaleraldehyde + H2O + oxidized benzyl viologen Vmax/Km is 54% compared to the value for hexanal Pyrococcus furiosus 2-methylvalerate + reduced benzyl viologen + H+
-
 ?
2-naphthaldehyde + H2O + oxidized benzyl viologen Vmax/Km is 6.7% compared to the value for hexanal Pyrococcus furiosus 2-naphthoate + reduced benzyl viologen + H+
-
 ?
3-phenylbutyraldehyde + H2O + oxidized benzyl viologen Vmax/Km is 11% compared to the value for hexanal Pyrococcus furiosus 3-phenylbutyrate + reduced benzyl viologen + H+
-
 ?
cinnamaldehyde + H2O + oxidized benzyl viologen Vmax/Km is 5.3% compared to the value for hexanal Pyrococcus furiosus cinnamate + reduced benzyl viologen + H+
-
 ?
hexanal + H2O + 2 oxidized ferredoxin
-
 Pyrococcus furiosus hexanoate + 2 reduced ferredoxin + 2 H+
-
 ?
hexanal + H2O + oxidized methyl viologen
-
 Pyrococcus furiosus hexanoate + reduced methyl viologen + H+
-
 ?
hydratropaldehyde + H2O + oxidized benzyl viologen Vmax/Km is 89% compared to the value for hexanal Pyrococcus furiosus ? + reduced benzyl viologen + H+
-
 ?
isobutyraldehyde + H2O + oxidized benzyl viologen Vmax/Km is 17% compared to the value for hexanal Pyrococcus furiosus isobutyrate + reduced benzyl viologen + H+
-
 ?
additional information ferredoxin can be the physiological electron acceptor Pyrococcus furiosus ?
-
 ?
additional information it is distinguished from aldehyde ferredoxin oxidoreductase (EC 1.2.7.5) by its low activity and affinity for crotonaldehyde, wich is one of the best substrates of aldehyde ferredoxin oxidoreductase. It is distinguished from formaldehyde ferredoxin oxidoreductase by its low activity and affinity for formaldehyde, which is one of the best substrates of formaldehyde ferredoxin oxidoreductase. It shows high affinity for several substituted and nonsubstituted aliphatic and aromatic aldehydes with various chain lengths. Very low activity (Vmax/Km is less than 5% compared to activity with hexanal): formaldehyde, crotonaldehyde, acetaldehyde, glutaraldehyde, 2-methoxybenzaldehyde. No activity with glyceraldehyde 3-phosphate Pyrococcus furiosus ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 67000, SDS-PAGE Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
WOR5
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Pyrococcus furiosus