BRENDA - Enzyme Database
show all sequences of 1.2.99.7

Subunit composition, crystallization and preliminary crystallographic studies of the Desulfovibrio gigas aldehyde oxidoreductase containing molybdenum and [2Fe-2S] centers

Romao, M.J.; Barata, B.A.; Archer, M.; Lobeck, K.; Moura, I.; Carrondo, M.A.; LeGall, J.; Lottspeich, F.; Huber, R.; Moura, J.J.; Eur. J. Biochem. 215, 729-732 (1993)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization (Commentary)
Organism
vapour-diffusion using sitting drops and a reservoir containing 100 mM Hepes, pH 7.5, 200 mM MgCl2 and 30% isopropanol, droplets are prepared by mixing 0.004 ml of a 13 mg/ml protein solution in 10 mM Tris, pH 7.6 with 0.002 ml reservoir solution, single crystals are obtained within 3-6 weeks at 4°C, crystals diffract to 3.0 A
Desulfovibrio gigas
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
[Fe-2S] cluster
Desulfovibrio gigas
Molybdenum
bound to a pterin cofactor and [2Fe-2S] cluster
Desulfovibrio gigas
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
100000
-
2 * 100000, SDS-PAGE
Desulfovibrio gigas
200000
-
gel filtration
Desulfovibrio gigas
Organism
Organism
UniProt
Commentary
Textmining
Desulfovibrio gigas
-
NCIB 9332
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Desulfovibrio gigas
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
acetaldehyde + H2O + electron acceptor
-
655397
Desulfovibrio gigas
acetate + reduced electron acceptor
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 100000, SDS-PAGE
Desulfovibrio gigas
Crystallization (Commentary) (protein specific)
Crystallization
Organism
vapour-diffusion using sitting drops and a reservoir containing 100 mM Hepes, pH 7.5, 200 mM MgCl2 and 30% isopropanol, droplets are prepared by mixing 0.004 ml of a 13 mg/ml protein solution in 10 mM Tris, pH 7.6 with 0.002 ml reservoir solution, single crystals are obtained within 3-6 weeks at 4°C, crystals diffract to 3.0 A
Desulfovibrio gigas
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
[Fe-2S] cluster
Desulfovibrio gigas
Molybdenum
bound to a pterin cofactor and [2Fe-2S] cluster
Desulfovibrio gigas
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
100000
-
2 * 100000, SDS-PAGE
Desulfovibrio gigas
200000
-
gel filtration
Desulfovibrio gigas
Purification (Commentary) (protein specific)
Commentary
Organism
-
Desulfovibrio gigas
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
acetaldehyde + H2O + electron acceptor
-
655397
Desulfovibrio gigas
acetate + reduced electron acceptor
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 100000, SDS-PAGE
Desulfovibrio gigas
Other publictions for EC 1.2.99.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740765
Correia
Aromatic aldehydes at the acti ...
Desulfovibrio gigas
J. Biol. Inorg. Chem.
20
219-229
2015
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4
8
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2
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9
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8
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4
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4
4
8
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9
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8
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8
8
740766
Gomez
Isotropic exchange interaction ...
Desulfovibrio gigas
J. Biol. Inorg. Chem.
20
233-242
2015
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741287
Marangon
Kinetic and structural studies ...
Desulfovibrio gigas
PLoS ONE
8
e83234
2013
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1
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725548
Maia
Nitrite reduction by xanthine ...
Desulfovibrio gigas
J. Biol. Inorg. Chem.
16
443-460
2011
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1
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712240
Santos-Silva
Kinetic, structural, and EPR s ...
Desulfovibrio gigas
J. Am. Chem. Soc.
131
7990-7998
2009
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1
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4
1
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1
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2
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1
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1
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1
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4
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1
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1
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1
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1
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1
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655476
Correia dos Santos
Direct electrochemistry of the ...
Desulfovibrio gigas
Eur. J. Biochem.
271
1329-1338
2004
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644705
Uchida
Purification and characterizat ...
Pseudomonas sp., Pseudomonas sp. KY 469
FEMS Microbiol. Lett.
229
31-36
2003
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1
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656294
Rebelo
Structure refinement of the al ...
Desulfovibrio gigas
J. Biol. Inorg. Chem.
6
791-800
2001
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1
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1
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1
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1
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644707
Andrade
Aldehyde oxidoreductase activi ...
Desulfovibrio alaskensis, Desulfovibrio alaskensis NCIMB 13491, Desulfovibrio alaskensis NCIMB 1349
Eur. J. Biochem.
267
2054-2061
2000
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1
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2
2
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4
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1
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1
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6
1
1
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1
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1
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2
2
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1
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1
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6
1
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1
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654573
Caldeira
Analysis of the electron param ...
Desulfovibrio gigas
Biochemistry
39
2700-2707
2000
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1
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1
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657173
Huber
A structure-based catalytic me ...
Desulfovibrio gigas
Proc. Natl. Acad. Sci. USA
93
8846-8851
1996
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11936
Hensgens
Purification and characterizat ...
Desulfovibrio gigas
J. Bacteriol.
177
6195-6200
1995
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4
5
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3
3
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1
1
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1
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1
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15
1
1
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1
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1
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4
-
5
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3
3
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1
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1
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1
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15
1
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1
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1
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655399
Thoenes
Molecular cloning and sequence ...
Desulfovibrio gigas
Eur. J. Biochem.
220
901-910
1994
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1
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1
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654563
Barata
Aldehyde oxidoreductase activi ...
Desulfovibrio gigas
Biochemistry
32
11559-11568
1993
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4
-
2
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1
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1
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1
1
10
-
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4
1
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4
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2
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1
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1
1
10
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4
1
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655397
Romao
Subunit composition, crystalli ...
Desulfovibrio gigas
Eur. J. Biochem.
215
729-732
1993
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1
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2
2
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1
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1
1
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1
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2
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1
1
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654473
Bray
Information from EPR spectrosc ...
Desulfovibrio gigas
Biochem. J.
280
817-820
1991
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2
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1
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1
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390386
Turner
The molybdenum iron-sulphur pr ...
Desulfovibrio gigas
Biochem. J.
243
755-761
1987
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