BRENDA - Enzyme Database show
show all sequences of 1.21.3.4

Enzymatic synthesis of (+)- and (-)-bisdechlorogeodin with sulochrin oxidase from Penicillium frequentans and Oospora sulphurea-ochracea

Nordlöv, H.; Gatenbeck, S.; Arch. Microbiol. 131, 208-211 (1982)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
not inhibited by 2 mM neocuproin, 5 mM, potassium cyanide or 10 mM EDTA
Penicillium glabrum
Sodium diethyldithiocarbamate
0.1 mM, 66% inhibition, 1 mM, complete inhibition
Penicillium glabrum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0065
-
sulochrin
-
Penicillium glabrum
0.063
-
O2
-
Penicillium glabrum
0.4
-
N,N-Dimethyl-p-phenylenediamine
-
Penicillium glabrum
1
-
N,N,N',N'-tetramethyl-p-phenylenediamine
-
Penicillium glabrum
2
-
p-phenylenediamine
-
Penicillium glabrum
5
-
o-phenylenediamine
-
Penicillium glabrum
40
-
catechol
-
Penicillium glabrum
60
-
hydroquinone
-
Penicillium glabrum
150
-
(+)-ascorbic acid
-
Penicillium glabrum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
intracellular
no extracellular activity
Penicillium glabrum
5622
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
copper
enzyme contains 6 copper atoms
Penicillium glabrum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27800
-
1 * 85000 + 1 * 27800, possible structure: 2 noncovalently bound identical subunits, each of which consists of 3 smaller subunits, SDS-PAGE
Penicillium glabrum
85000
-
1 * 85000 + 1 * 27800, possible structure: 2 noncovalently bound identical subunits, each of which consists of 3 smaller subunits, SDS-PAGE
Penicillium glabrum
157000
-
gel filtration
Penicillium glabrum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sulochrin + O2
Penicillium glabrum
main function: synthesis of bisdechlorogeodin, involved in biosynthesis of mold metabolites related to the antibiotic griseofulvin
(+)-bisdechlorogeodin + H2O
-
Penicillium glabrum
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Penicillium glabrum
-
-
-
Penicillium glabrum CMI 96659
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
19.5% carbohydrate
Penicillium glabrum
Purification (Commentary)
Commentary
Organism
ammonium sulfate, pH 3.3, isoelectric focusing, gel filtration
Penicillium glabrum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Penicillium glabrum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1
-
-
Penicillium glabrum
Storage Stability
Storage Stability
Organism
-20°C, pH 5.8, citrate-phosphate buffer, several months, no loss in activity
Penicillium glabrum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-ascorbic acid + O2
-
437825
Penicillium glabrum
?
-
-
-
?
catechol + O2
-
437825
Penicillium glabrum
?
-
-
-
?
catechol + O2
-
437825
Penicillium glabrum CMI 96659
?
-
-
-
?
hydroquinone + O2
-
437825
Penicillium glabrum
?
-
-
-
?
hydroquinone + O2
-
437825
Penicillium glabrum CMI 96659
?
-
-
-
?
N,N,N',N'-tetramethyl-p-phenylenediamine + O2
-
437825
Penicillium glabrum
?
-
-
-
?
N,N,N',N'-tetramethyl-p-phenylenediamine + O2
-
437825
Penicillium glabrum CMI 96659
?
-
-
-
?
N,N-dimethyl-p-phenylenediamine + O2
-
437825
Penicillium glabrum
?
-
-
-
?
N,N-dimethyl-p-phenylenediamine + O2
-
437825
Penicillium glabrum CMI 96659
?
-
-
-
?
o-phenylenediamine + O2
-
437825
Penicillium glabrum
?
-
-
-
?
p-phenylenediamine + O2
-
437825
Penicillium glabrum
?
-
-
-
?
p-phenylenediamine + O2
-
437825
Penicillium glabrum CMI 96659
?
-
-
-
?
sulochrin + O2
stereospecific formation of (+)-isomer
437825
Penicillium glabrum
(+)-bisdechlorogeodin + H2O
-
437825
Penicillium glabrum
?
sulochrin + O2
main function: synthesis of bisdechlorogeodin, involved in biosynthesis of mold metabolites related to the antibiotic griseofulvin
437825
Penicillium glabrum
(+)-bisdechlorogeodin + H2O
-
437825
Penicillium glabrum
?
Subunits
Subunits
Commentary
Organism
dimer
1 * 85000 + 1 * 27800, possible structure: 2 noncovalently bound identical subunits, each of which consists of 3 smaller subunits, SDS-PAGE
Penicillium glabrum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Penicillium glabrum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.8
-
-
Penicillium glabrum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
3.5
7
-
Penicillium glabrum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
not inhibited by 2 mM neocuproin, 5 mM, potassium cyanide or 10 mM EDTA
Penicillium glabrum
Sodium diethyldithiocarbamate
0.1 mM, 66% inhibition, 1 mM, complete inhibition
Penicillium glabrum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0065
-
sulochrin
-
Penicillium glabrum
0.063
-
O2
-
Penicillium glabrum
0.4
-
N,N-Dimethyl-p-phenylenediamine
-
Penicillium glabrum
1
-
N,N,N',N'-tetramethyl-p-phenylenediamine
-
Penicillium glabrum
2
-
p-phenylenediamine
-
Penicillium glabrum
5
-
o-phenylenediamine
-
Penicillium glabrum
40
-
catechol
-
Penicillium glabrum
60
-
hydroquinone
-
Penicillium glabrum
150
-
(+)-ascorbic acid
-
Penicillium glabrum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
intracellular
no extracellular activity
Penicillium glabrum
5622
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
copper
enzyme contains 6 copper atoms
Penicillium glabrum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27800
-
1 * 85000 + 1 * 27800, possible structure: 2 noncovalently bound identical subunits, each of which consists of 3 smaller subunits, SDS-PAGE
Penicillium glabrum
85000
-
1 * 85000 + 1 * 27800, possible structure: 2 noncovalently bound identical subunits, each of which consists of 3 smaller subunits, SDS-PAGE
Penicillium glabrum
157000
-
gel filtration
Penicillium glabrum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sulochrin + O2
Penicillium glabrum
main function: synthesis of bisdechlorogeodin, involved in biosynthesis of mold metabolites related to the antibiotic griseofulvin
(+)-bisdechlorogeodin + H2O
-
Penicillium glabrum
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
19.5% carbohydrate
Penicillium glabrum
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate, pH 3.3, isoelectric focusing, gel filtration
Penicillium glabrum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Penicillium glabrum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1
-
-
Penicillium glabrum
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, pH 5.8, citrate-phosphate buffer, several months, no loss in activity
Penicillium glabrum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-ascorbic acid + O2
-
437825
Penicillium glabrum
?
-
-
-
?
catechol + O2
-
437825
Penicillium glabrum
?
-
-
-
?
catechol + O2
-
437825
Penicillium glabrum CMI 96659
?
-
-
-
?
hydroquinone + O2
-
437825
Penicillium glabrum
?
-
-
-
?
hydroquinone + O2
-
437825
Penicillium glabrum CMI 96659
?
-
-
-
?
N,N,N',N'-tetramethyl-p-phenylenediamine + O2
-
437825
Penicillium glabrum
?
-
-
-
?
N,N,N',N'-tetramethyl-p-phenylenediamine + O2
-
437825
Penicillium glabrum CMI 96659
?
-
-
-
?
N,N-dimethyl-p-phenylenediamine + O2
-
437825
Penicillium glabrum
?
-
-
-
?
N,N-dimethyl-p-phenylenediamine + O2
-
437825
Penicillium glabrum CMI 96659
?
-
-
-
?
o-phenylenediamine + O2
-
437825
Penicillium glabrum
?
-
-
-
?
p-phenylenediamine + O2
-
437825
Penicillium glabrum
?
-
-
-
?
p-phenylenediamine + O2
-
437825
Penicillium glabrum CMI 96659
?
-
-
-
?
sulochrin + O2
stereospecific formation of (+)-isomer
437825
Penicillium glabrum
(+)-bisdechlorogeodin + H2O
-
437825
Penicillium glabrum
?
sulochrin + O2
main function: synthesis of bisdechlorogeodin, involved in biosynthesis of mold metabolites related to the antibiotic griseofulvin
437825
Penicillium glabrum
(+)-bisdechlorogeodin + H2O
-
437825
Penicillium glabrum
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
1 * 85000 + 1 * 27800, possible structure: 2 noncovalently bound identical subunits, each of which consists of 3 smaller subunits, SDS-PAGE
Penicillium glabrum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Penicillium glabrum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.8
-
-
Penicillium glabrum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
3.5
7
-
Penicillium glabrum
Other publictions for EC 1.21.3.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
437827
Huang
Purification and characterizat ...
Penicillium glabrum, Penicillium glabrum CMI 96659
Biol. Pharm. Bull.
19
42-46
1996
-
-
-
-
-
1
1
-
-
1
2
-
-
4
-
-
1
-
-
-
1
-
6
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
2
-
-
-
-
1
-
-
1
-
6
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
437825
Nordlöv
Enzymatic synthesis of (+)- an ...
Penicillium glabrum, Penicillium glabrum CMI 96659
Arch. Microbiol.
131
208-211
1982
-
-
-
-
-
-
2
9
1
1
3
1
-
5
-
1
1
-
-
1
1
1
14
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
9
1
1
3
1
-
-
1
1
-
1
1
1
14
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-