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Literature summary for 1.21.3.8 extracted from
- Purification and characterization of cannabidiolic-acid synthase from Cannabis sativa L.. Biochemical analysis of a novel enzyme that catalyzes the oxidocyclization of cannabigerolic acid to cannabidiolic acid (1996), J. Biol. Chem., 271, 17411-17416.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | complete inhibition at 2 mM | Cannabis sativa |  |
| additional information | EDTA has little influence on the enzyme activity at concentrations up to 5 mM | Cannabis sativa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.137 | - |
cannabigerolate | in 100 mM sodium citrate buffer (pH 5.0), at 30°C | Cannabis sativa | |
| 0.206 | - |
cannabinerolate | in 100 mM sodium citrate buffer (pH 5.0), at 30°C | Cannabis sativa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme does not require metal ions | Cannabis sativa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74000 | - |
1 * 74000, SDS-PAGE | Cannabis sativa |
| 75000 | - |
gel filtration | Cannabis sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cannabis sativa | - |
CBDA and Mexican strains | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-cellulose column chromatography, phenyl-Sepharose CL-4B column chromatography, and hydroxylapatite column chromatography | Cannabis sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Cannabis sativa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.175 | - |
enzyme from supernatant, in 100 mM sodium citrate buffer (pH 5.0), at 30°C | Cannabis sativa |
| 9 | - |
after 519fold purification, in 100 mM sodium citrate buffer (pH 5.0), at 30°C | Cannabis sativa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 40% (v/v) glycerol, ineffective storage conditions for enzyme stabilization | Cannabis sativa |
| 4°C, purified enzyme in 10 mM sodium phosphate buffer (pH 7.0), 3 mM beta-mercaptoethanol, 2 weeks, 10-20% loss of activity | Cannabis sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cannabigerolate + O2 | best substrate | Cannabis sativa | cannabidiolate + H2O2 | - |
? | |
| cannabinerolate + O2 | - |
Cannabis sativa | cannabidiolate + H2O2 | - |
? | |
| additional information | the enzyme does not require molecular oxygen for the production of cannabidiolate in the presence or absence of NADPH | Cannabis sativa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 74000, SDS-PAGE | Cannabis sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CBDA synthase | - |
Cannabis sativa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
cannabinerolate | in 100 mM sodium citrate buffer (pH 5.0), at 30°C | Cannabis sativa | |
| 0.19 | - |
cannabigerolate | in 100 mM sodium citrate buffer (pH 5.0), at 30°C | Cannabis sativa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Cannabis sativa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | half-maximal activities at pH values around 4.0 and 6.0 | Cannabis sativa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme does not require any coenzyme like NAD+, NADP+, FAD, and FMN | Cannabis sativa |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Cannabis sativa | isoelectric focusing | - |
6.1 |
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