Literature summary for 1.21.4.5 extracted from

Habash, M.B.; Beaudette, L.A.; Cassidy, M.B.; Leung, K.T.; Hoang, T.A.; Vogel, H.J.; Trevors, J.T.; Lee, H.
Characterization of tetrachlorohydroquinone reductive dehalogenase from Sphingomonas sp. UG30 (2002), Biochem. Biophys. Res. Commun., 299, 634-640 .

Search for more literature for 1.21.4.5

Activating Compound

Activating Compound	Comment	Organism	Structure
dithiothreitol	increasing DTT concentration results in increasing PcpC activity, reaching a maximum at approximately 30 mM DTT and remaining high up to 100 mM DTT	Sphingobium sp. UG30

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Sphingobium sp. UG30

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	PcpC is not inhibited by the 2,3,5,6-tetrachlorohydroquinone substrate at concentrations 0.3 mM	Sphingobium sp. UG30

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0203	-	2,3,5,6-tetrachlorohydroquinone	pH 8.7, 50°C	Sphingobium sp. UG30
0.1492	-	glutathione	pH 8.7, 50°C	Sphingobium sp. UG30

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28245	-	calculated from sequence	Sphingobium sp. UG30
30000	-	SDS-PAGE	Sphingobium sp. UG30
56000	-	gel filtration	Sphingobium sp. UG30

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2,3,5,6-tetrachlorohydroquinone + 2 glutathione	Sphingobium sp. UG30	-	2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide	-	?
2,3,6-trichlorohydroquinone + 2 glutathione	Sphingobium sp. UG30	-	2,6-dichlorohydroquinone + Cl- + glutathione disulfide	-	?

Organism

Organism	UniProt	Comment	Textmining
Sphingobium sp. UG30	Q937X0	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Sphingobium sp. UG30

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
103	-	pH 8.7, 50°C	Sphingobium sp. UG30

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,3,5,6-tetrachlorohydroquinone + 2 glutathione	-	Sphingobium sp. UG30	2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide	-	?
2,3,6-trichlorohydroquinone + 2 glutathione	-	Sphingobium sp. UG30	2,6-dichlorohydroquinone + Cl- + glutathione disulfide	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 30000, SDS-PAGE	Sphingobium sp. UG30

Synonyms

Synonyms	Comment	Organism
pcpC	-	Sphingobium sp. UG30
tetrachlorohydroquinone reductive dehalogenase	-	Sphingobium sp. UG30

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Sphingobium sp. UG30

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	50	30°C: about 30% of maximal activity, 50°C: about 60% of maximal activity	Sphingobium sp. UG30

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
100	-	10 min, complete inactivation	Sphingobium sp. UG30

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
48.5	-	2,3,5,6-tetrachlorohydroquinone	pH 8.7, 50°C	Sphingobium sp. UG30

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.7	-	-	Sphingobium sp. UG30

pH Range

pH Minimum	pH Maximum	Comment	Organism
8	9.5	pH 8.0: about 50% of maximal activity, pH 9.5: about 40% of maximal activity	Sphingobium sp. UG30

pI Value

Organism	Comment	pI Value Maximum	pI Value
Sphingobium sp. UG30	calculated from sequence	-	4.99

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2389	-	2,3,5,6-tetrachlorohydroquinone	pH 8.7, 50°C	Sphingobium sp. UG30

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)