Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sphingobium sp. UG30 |
Protein Variants | Comment | Organism |
---|---|---|
C14A | the mutant enzyme displays greater than 25fold decreased activity relative to the wild-type enzyme. The pH optimum decreases from 50°C for the wild-type enzyme to 44°C for mutant enzyme S12A | Sphingobium sp. UG30 |
S12A | the mutant enzyme displays greater than 25fold decreased activity relative to the wild-type enzyme, the mutant enzyme shows an increase in the molar ellipticity between 260 and 280 nm, relative to the wild-type enzyme. The pH optimum decreases from 50°C for the wild-type enzyme to 44°C for mutant enzyme S12A | Sphingobium sp. UG30 |
S15A | the mutant enzyme displays greater than 25fold decreased activity relative to the wild-type enzyme. The pH optimum decreases from 50°C for the wild-type enzyme to 40°C for mutant enzyme S12A | Sphingobium sp. UG30 |
Y7F | activity of the mutant enzyme is not significantly different from the wild-type enzyme | Sphingobium sp. UG30 |
Y9F | activity of the mutant enzyme is not significantly different from the wild-type enzyme | Sphingobium sp. UG30 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3,5,6-tetrachlorohydroquinone | mutant enzymes S15A and S12A displayed substrate inhibition above 0.035 mM | Sphingobium sp. UG30 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.4 | - |
2,3,5,6-tetrachlorohydroquinone | pH 8.7, 37°, wild-type enzyme | Sphingobium sp. UG30 | |
16 | - |
2,3,5,6-tetrachlorohydroquinone | pH 8.7, 37°C, mutant enzyme C14A | Sphingobium sp. UG30 | |
31.1 | - |
2,3,5,6-tetrachlorohydroquinone | pH 8.7, 37°C, mutant enzyme S12A | Sphingobium sp. UG30 | |
103 | - |
glutathione | pH 8.7, 37°C, mutant enzyme S12A | Sphingobium sp. UG30 | |
188 | - |
glutathione | pH 8.7, 37°C, wild-type enzyme | Sphingobium sp. UG30 | |
527 | - |
glutathione | pH 8.7, 37°C, mutant enzyme S15A | Sphingobium sp. UG30 | |
790 | - |
glutathione | pH 8.7, 37°C, mutant enzyme C14A | Sphingobium sp. UG30 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
SDS-PAGE | Sphingobium sp. UG30 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3,5,6-tetrachlorohydroquinone + 2 glutathione | Sphingobium sp. UG30 | - |
2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide | - |
? | |
2,3,6-trichlorohydroquinone + 2 glutathione | Sphingobium sp. UG30 | - |
2,6-dichlorohydroquinone + Cl- + glutathione disulfide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium sp. UG30 | Q937X0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sphingobium sp. UG30 |
Storage Stability | Organism |
---|---|
-20°C, enzyme activity is retained during storage under these conditions for at least 6 weeks | Sphingobium sp. UG30 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3,5,6-tetrachlorohydroquinone + 2 glutathione | - |
Sphingobium sp. UG30 | 2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide | - |
? | |
2,3,6-trichlorohydroquinone + 2 glutathione | - |
Sphingobium sp. UG30 | 2,6-dichlorohydroquinone + Cl- + glutathione disulfide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, SDS-PAGE | Sphingobium sp. UG30 |
Synonyms | Comment | Organism |
---|---|---|
pcpC | - |
Sphingobium sp. UG30 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
mutant enzyme S15A | Sphingobium sp. UG30 |
44 | - |
mutant enzyme S12A and mutant enzyme C14A | Sphingobium sp. UG30 |
50 | - |
wild-type enzyme | Sphingobium sp. UG30 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
2,3,5,6-tetrachlorohydroquinone | pH 8.7, 37°C, mutant enzyme C14A | Sphingobium sp. UG30 | |
6.3 | - |
2,3,5,6-tetrachlorohydroquinone | pH 8.7, 37°C, mutant enzyme S12A | Sphingobium sp. UG30 | |
9.6 | - |
2,3,5,6-tetrachlorohydroquinone | pH 8.7, 37°, wild-type enzyme | Sphingobium sp. UG30 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.087 | - |
2,3,5,6-tetrachlorohydroquinone | pH 8.7, 37°C, mutant enzyme C14A | Sphingobium sp. UG30 | |
0.204 | - |
2,3,5,6-tetrachlorohydroquinone | pH 8.7, 37°C, mutant enzyme S12A | Sphingobium sp. UG30 | |
0.839 | - |
2,3,5,6-tetrachlorohydroquinone | pH 8.7, 37°, wild-type enzyme | Sphingobium sp. UG30 |