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Literature summary for 1.21.98.3 extracted from

  • Wiesselmann, M.; Hebecker, S.; Borrero-de Acuna, J.M.; Nimtz, M.; Bollivar, D.; Jaensch, L.; Moser, J.; Jahn, D.
    Mg-protoporphyrin IX monomethyl ester cyclase from Rhodobacter capsulatus radical SAM-dependent synthesis of the isocyclic ring of bacteriochlorophylls (2020), Biochem. J., 477, 4635-4654 .
    View publication on PubMed
Search for more literature for 1.21.98.3

Activating Compound

Activating Compound Comment Organism Structure
additional information BchE activity is stimulated by increasing concentrations of NADPH or SAM Rhodobacter capsulatus
NADPH
-
 Rhodobacter capsulatus
S-adenosyl-L-methionine
-
 Rhodobacter capsulatus

Inhibitors

Inhibitors Comment Organism Structure
S-adenosyl-L-homocysteine
-
 Rhodobacter capsulatus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Rhodobacter capsulatus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O Rhodobacter capsulatus overall reaction 3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
 ?
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O Rhodobacter capsulatus NBRC 16581 overall reaction 3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
 ?
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O Rhodobacter capsulatus ATCC BAA-309 overall reaction 3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
 ?
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O Rhodobacter capsulatus SB1003 overall reaction 3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus P26168
-
-
Rhodobacter capsulatus ATCC BAA-309 P26168
-
-
Rhodobacter capsulatus NBRC 16581 P26168
-
-
Rhodobacter capsulatus SB1003 P26168
-
-

Reaction

Reaction Comment Organism Reaction ID
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O = 3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine the oxygen-independent conversion of Mg-protoporphyrin IX monomethyl ester (Mg-PME) to protochlorophyllide (Pchlide) is catalyzed by the anaerobic Mg-PME cyclase termed BchE, via an unusual radical S-adenosylmethionine (SAM) and cobalamin-dependent BchE catalysis Rhodobacter capsulatus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O overall reaction Rhodobacter capsulatus 3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
 ?
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O overall reaction Rhodobacter capsulatus NBRC 16581 3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
 ?
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O overall reaction Rhodobacter capsulatus ATCC BAA-309 3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
 ?
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O overall reaction Rhodobacter capsulatus SB1003 3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
 ?
additional information establishment of in vitro biosynthesis of the isocyclic ring moiety of bacteriochlorophyll using purified recombinant BchE, method development, overview Rhodobacter capsulatus ?
-
-
additional information establishment of in vitro biosynthesis of the isocyclic ring moiety of bacteriochlorophyll using purified recombinant BchE, method development, overview Rhodobacter capsulatus NBRC 16581 ?
-
-
additional information establishment of in vitro biosynthesis of the isocyclic ring moiety of bacteriochlorophyll using purified recombinant BchE, method development, overview Rhodobacter capsulatus ATCC BAA-309 ?
-
-
additional information establishment of in vitro biosynthesis of the isocyclic ring moiety of bacteriochlorophyll using purified recombinant BchE, method development, overview Rhodobacter capsulatus SB1003 ?
-
-

Synonyms

Synonyms Comment Organism
BChE
-
 Rhodobacter capsulatus
Mg-protoporphyrin IX monomethyl ester cyclase
-
 Rhodobacter capsulatus

Cofactor

Cofactor Comment Organism Structure
Cobalamin dependent on, ligated via Asp248, Glu249, Leu29, Thr71, Val97 Rhodobacter capsulatus
additional information membrane-localized BchE requires an additional, heat-sensitive cytosolic cobalamin component for activity, BchE catalysis is not sustained in chimeric experiments when a cytosolic extract from Escherichia coli is used as a substitute Rhodobacter capsulatus
S-adenosyl-L-methionine dependent on, ligated via Phe210, Glu308 and Lys320 Rhodobacter capsulatus
[4Fe-4S] cluster ligated viaCys204, Cys208, Cys211 Rhodobacter capsulatus

General Information

General Information Comment Organism
physiological function during bacteriochlorophyll a biosynthesis, the oxygen-independent conversion of Mg-protoporphyrin IX monomethyl ester (Mg-PME) to protochlorophyllide (Pchlide) is catalyzed by the anaerobic Mg-PME cyclase termed BchE, via an unusual radical S-adenosylmethionine (SAM) and cobalamin-dependent BchE catalysis. Membrane-localized BchE requires an additional, heat-sensitive cytosolic component for activity Rhodobacter capsulatus