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Literature summary for 1.21.99.3 extracted from
- Iodothyronine deiodinases (2002), Methods Enzymol., 347, 125-167.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 3,3',5-triiodo-L-thyronine | induces enzyme | vertebrata |  |
| Epidermal growth factor | - |
Rattus norvegicus | |
| Epidermal growth factor | induces enzyme | vertebrata | |
| fibroblast growth factor | induces enzyme | vertebrata | |
| additional information | expression and function of 5-deiodinase are sensitive to thyroid hormone status, various cytokines and growth factors, severe illness, reactive oxygen species, a variety of hormones and signaling compounds, circadian rythm, and pharmacological agents | Homo sapiens |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | expression and function of the 5-deiodinase and 5-deiodinase isozymes are sensitive to thyroid hormone status, various cytokines and growth factors, severe illness, reactive oxygen species, a variety of hormones and signaling compounds, circadian rythm, and pharmacological agents, and therefore might be useful in sensor function of physiology and pathophysiology | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iopanoic acid | - |
Homo sapiens | |
| iopanoic acid | - |
vertebrata | |
| additional information | expression and function of the 5-deiodinase and 5-deiodinase isozymes are sensitive to thyroid hormone status, various cytokines and growth factors, severe illness, reactive oxygen species, a variety of hormones and signaling compounds, circadian rythm, and pharmacological agents, and therefore might be useful in sensor function of physiology and pathophysiology | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | review on enzyme and 5-deiodinase isoforms type I and II | Homo sapiens | |
| additional information | - |
additional information | review on enzyme and 5-deiodinase isoforms type I and II | Rattus norvegicus | |
| additional information | - |
additional information | review on enzyme and 5-deiodinase isoforms type I and II | vertebrata |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Homo sapiens | - |
- |
| additional information | membrane association is not essential for maintenance of functional activity | Rattus norvegicus | - |
- |
| additional information | subcellular localization of the different isozyme types in different tissues, overview | vertebrata | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,3',5-triiodo-L-thyronine + AH2 | Mus musculus | - |
3,3'-diiodo-L-thyronine + iodide + A + H+ | hormonally inactive | ? | |
| 3,3',5-triiodo-L-thyronine + AH2 | Homo sapiens | - |
3,3'-diiodo-L-thyronine + iodide + A + H+ | hormonally inactive | ? | |
| 3,3',5-triiodo-L-thyronine + AH2 | Rattus norvegicus | - |
3,3'-diiodo-L-thyronine + iodide + A + H+ | hormonally inactive | ? | |
| 3,3',5-triiodo-L-thyronine + AH2 | vertebrata | - |
3,3'-diiodo-L-thyronine + iodide + A + H+ | hormonally inactive | ? | |
| L-thyroxine + AH2 | Mus musculus | enzyme III performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | hormonally inactive | ? | |
| L-thyroxine + AH2 | Homo sapiens | enzyme III performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | hormonally inactive | ? | |
| L-thyroxine + AH2 | vertebrata | enzyme III performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | hormonally inactive | ? | |
| L-thyroxine + AH2 | Rattus norvegicus | enzyme performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | hormonally inactive | ? | |
| additional information | Rattus norvegicus | enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content | ? | - |
? | |
| additional information | Mus musculus | isozyme type III performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine and enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content | ? | - |
? | |
| additional information | Homo sapiens | isozyme type III performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine and enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content | ? | - |
? | |
| additional information | vertebrata | isozyme type III performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine and enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| vertebrata | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 3,3',5'-triiodo-L-thyronine + iodide + acceptor + H+ = L-thyroxine + reduced acceptor | both 5'-deiodinase isoforms and 5-deiodinase contain a selenocysteine residue at the active site | vertebrata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Mus musculus | - |
| brain | - |
Homo sapiens | - |
| brain | - |
Rattus norvegicus | - |
| brain | - |
vertebrata | - |
| additional information | not: kidney, healthy adult liver, pituitary gland, thyroid gland | Mus musculus | - |
| additional information | not: kidney, healthy adult liver, pituitary gland, thyroid gland | Homo sapiens | - |
| additional information | not: kidney, healthy adult liver, pituitary gland, thyroid gland | vertebrata | - |
| placenta | - |
Mus musculus | - |
| placenta | - |
Homo sapiens | - |
| placenta | - |
Rattus norvegicus | - |
| placenta | - |
vertebrata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
overview on assay methods | Mus musculus |
| additional information | - |
overview on assay methods | Rattus norvegicus |
| additional information | - |
overview on assay methods | vertebrata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,3',5-triiodo-L-thyronine + AH2 | - |
Mus musculus | 3,3'-diiodo-L-thyronine + iodide + A + H+ | hormonally inactive | ? | |
| 3,3',5-triiodo-L-thyronine + AH2 | - |
Homo sapiens | 3,3'-diiodo-L-thyronine + iodide + A + H+ | hormonally inactive | ? | |
| 3,3',5-triiodo-L-thyronine + AH2 | - |
Rattus norvegicus | 3,3'-diiodo-L-thyronine + iodide + A + H+ | hormonally inactive | ? | |
| 3,3',5-triiodo-L-thyronine + AH2 | - |
Bos taurus | 3,3'-diiodo-L-thyronine + iodide + A + H+ | hormonally inactive | ? | |
| 3,3',5-triiodo-L-thyronine + AH2 | - |
vertebrata | 3,3'-diiodo-L-thyronine + iodide + A + H+ | hormonally inactive | ? | |
| L-thyroxine + AH2 | enzyme III performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine | Mus musculus | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | hormonally inactive | ? | |
| L-thyroxine + AH2 | enzyme III performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine | Homo sapiens | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | hormonally inactive | ? | |
| L-thyroxine + AH2 | enzyme III performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine | vertebrata | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | hormonally inactive | ? | |
| L-thyroxine + AH2 | enzyme performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine | Rattus norvegicus | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | hormonally inactive | ? | |
| L-thyroxine + AH2 | enzyme performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine and plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content | Mus musculus | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | - |
? | |
| L-thyroxine + AH2 | enzyme performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine and plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content | Homo sapiens | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | - |
? | |
| L-thyroxine + AH2 | enzyme performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine and plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content | Rattus norvegicus | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | - |
? | |
| L-thyroxine + AH2 | enzyme performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine and plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content | Bos taurus | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | - |
? | |
| L-thyroxine + AH2 | enzyme performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine and plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content | vertebrata | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | - |
? | |
| additional information | enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content | Rattus norvegicus | ? | - |
? | |
| additional information | isozyme type III performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine and enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content | Mus musculus | ? | - |
? | |
| additional information | isozyme type III performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine and enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content | Homo sapiens | ? | - |
? | |
| additional information | isozyme type III performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine and enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content | vertebrata | ? | - |
? | |
| additional information | selenium-level of cells influences the enzyme activity | Homo sapiens | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Rattus norvegicus |
| 37 | - |
assay at | Bos taurus |
| 37 | - |
assay at | vertebrata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
| 8 | - |
assay at | Bos taurus |
| 8 | - |
assay at | vertebrata |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| dithioerythritol | 5-20 mM, can be substituted by dithiothreititol | vertebrata | |
| dithioerythritol | 5-20 mM, can be substituted by dithiothreitol | Rattus norvegicus | |
| dithiothreitol | - |
Homo sapiens | |
| dithiothreitol | 5-20 mM, can be substituted by dithioerythritol | Rattus norvegicus | |
| dithiothreitol | 5-20 mM, can be substituted by dithioerythritol | vertebrata | |
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Release 2023.1 (January 2023)
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