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Literature summary for 1.21.99.3 extracted from

  • Schoenmakers, C.H.; Pigmans, I.G.; Kaptein, E.; Darras, V.M.; Visser, T.J.
    Reaction of the type III iodothyronine deiodinase with the affinity label N-bromoacetyl-triiodothyronine (1993), FEBS Lett., 335, 104-108.
    View publication on PubMed
Search for more literature for 1.21.99.3

Inhibitors

Inhibitors Comment Organism Structure
N-bromoacetyl-3,3',5-triiodo-L-thyronine irreversible inactivation; isozyme type III; reacts with the selenocysteine residue in the active site Gallus gallus
N-bromoacetyl-3,3',5-triiodo-L-thyronine irreversible inactivation; isozyme type III; reacts with the selenocysteine residue in the active site Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000005
-
 3,3',5-triiodo-L-thyronine pH 7.2, 37°C Gallus gallus
0.000005
-
 3,3',5-triiodo-L-thyronine pH 7.2, 37°C Rattus norvegicus
0.000005
-
 3,3',5-triiodo-L-thyronine isozyme type III Gallus gallus
0.000005
-
 3,3',5-triiodo-L-thyronine isozyme type III Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome isozyme type III Gallus gallus
-
-
microsome isozyme type III Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,3',5-triiodo-L-thyronine + AH2 Gallus gallus isozyme type III 3,3'-diiodo-L-thyronine + iodide + A + H+ hormonally inactive ?
3,3',5-triiodo-L-thyronine + AH2 Rattus norvegicus isozyme type III 3,3'-diiodo-L-thyronine + iodide + A + H+ hormonally inactive ?
3,3',5-triiodo-L-thyronine + AH2 Gallus gallus isozyme type III 3,3'-diiodo-L-thyronine + iodide + A + H+ i.e. 3,3'-diiodo-L-thyronine ?
3,3',5-triiodo-L-thyronine + AH2 Rattus norvegicus isozyme type III 3,3'-diiodo-L-thyronine + iodide + A + H+ i.e. 3,3'-diiodo-L-thyronine ?
L-thyroxine + AH2 Gallus gallus 5-deiodinase activity 3,3',5'-triiodo-L-thyronine + iodide + A + H+ hormonally inactive ?
L-thyroxine + AH2 Rattus norvegicus 5-deiodinase activity 3,3',5'-triiodo-L-thyronine + iodide + A + H+ hormonally inactive ?
additional information Gallus gallus enzyme performs inactivation of thyroid hormones ?
-
 ?
additional information Rattus norvegicus enzyme performs inactivation of thyroid hormones ?
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 isozyme type III
-
Rattus norvegicus
-
 Wistar rats
-
Rattus norvegicus
-
 isozyme type III
-

Source Tissue

Source Tissue Comment Organism Textmining
brain isozyme type III Gallus gallus
-
brain isozyme type III Rattus norvegicus
-
brain more abundant in fetal than in adult rat brain Rattus norvegicus
-
embryo
-
 Gallus gallus
-
liver embryonic Gallus gallus
-
liver isozyme type III Gallus gallus
-
liver not in fetal liver microsomes Rattus norvegicus
-
placenta isozyme type III Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,3',5-triiodo-L-thyronine + AH2 isozyme type III Gallus gallus 3,3'-diiodo-L-thyronine + iodide + A + H+ hormonally inactive ?
3,3',5-triiodo-L-thyronine + AH2 isozyme type III Rattus norvegicus 3,3'-diiodo-L-thyronine + iodide + A + H+ hormonally inactive ?
3,3',5-triiodo-L-thyronine + AH2 isozyme type III Gallus gallus 3,3'-diiodo-L-thyronine + iodide + A + H+ i.e. 3,3'-diiodo-L-thyronine ?
3,3',5-triiodo-L-thyronine + AH2 isozyme type III Rattus norvegicus 3,3'-diiodo-L-thyronine + iodide + A + H+ i.e. 3,3'-diiodo-L-thyronine ?
L-thyroxine + AH2 isozyme type III Gallus gallus 3,3',5'-triiodo-L-thyronine + iodide + A + H+
-
 ?
L-thyroxine + AH2 isozyme type III Rattus norvegicus 3,3',5'-triiodo-L-thyronine + iodide + A + H+
-
 ?
L-thyroxine + AH2 5-deiodinase activity Gallus gallus 3,3',5'-triiodo-L-thyronine + iodide + A + H+ hormonally inactive ?
L-thyroxine + AH2 5-deiodinase activity Rattus norvegicus 3,3',5'-triiodo-L-thyronine + iodide + A + H+ hormonally inactive ?
additional information enzyme performs inactivation of thyroid hormones Gallus gallus ?
-
 ?
additional information enzyme performs inactivation of thyroid hormones Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
More the N-bromoacetyl-3,3',5-triiodo-L-thyronine labeled 32 kDa protein is not isozyme type III, labeling and activity are not corresponding Gallus gallus
More the N-bromoacetyl-3,3',5-triiodo-L-thyronine labeled 32 kDa protein is not isozyme type III, labeling and activity are not corresponding Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Gallus gallus
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Gallus gallus
7.2
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
dithiothreitol i.e. DTT Gallus gallus
dithiothreitol i.e. DTT Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000005
-
 N-bromoacetyl-3,3',5-triiodo-L-thyronine pH 7.2, 37°C Rattus norvegicus
0.000005
-
 N-bromoacetyl-3,3',5-triiodo-L-thyronine isozyme type III Rattus norvegicus