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Literature summary for 1.21.99.5 extracted from
- The proton/electron ratio of the menaquinone-dependent electron transport from dihydrogen to tetrachloroethene in "Dehalobacter restrictus" (1996), J. Bacteriol., 178, 2328-2333.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | degradation of lipophilic solvent tetrachloroethene, one of the most abundant halogenated xenobiotic pollutants in the environment | Dehalobacter restrictus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Propyl iodide | 1-iodopropane; 1-iodopropane, photoreversible inactivation | Dehalobacter restrictus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Dehalobacter restrictus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasmic membrane | cytoplasmic membrane | Dehalobacter restrictus | - |
- |
| cytoplasmic membrane | cytoplasmic side of membrane, mainly associated with the membrane | Dehalobacter restrictus | - |
- |
| additional information | cellular localization | Dehalobacter restrictus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cobalt | - |
Dehalobacter restrictus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tetrachloroethene + reduced acceptor | Dehalobacter restrictus | - |
trichloroethene + chloride + acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dehalobacter restrictus | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor | mechanism | Dehalobacter restrictus | |
| trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor | thermodynamics | Dehalobacter restrictus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.33 | - |
- |
Dehalobacter restrictus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tetrachloroethene + reduced acceptor | - |
Dehalobacter restrictus | trichloroethene + chloride + acceptor | - |
? | |
| tetrachloroethene + reduced acceptor | lipophilic solvent tetrachloroethene, one of the most abundant halogenated xenobiotic pollutants in the environment | Dehalobacter restrictus | trichloroethene + chloride + acceptor | - |
? | |
| tetrachloroethene + reduced acceptor | perchloroethylene, perchloroethene, PCE | Dehalobacter restrictus | trichloroethene + chloride + acceptor | - |
? | |
| trichloroethene + reduced acceptor | - |
Dehalobacter restrictus | cis-1,2-dichloroethene + chloride + acceptor | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Dehalobacter restrictus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
assay at | Dehalobacter restrictus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| corrinoid | - |
Dehalobacter restrictus | |
| menaquinone | membrane-bound menaquinone | Dehalobacter restrictus | |
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