Literature summary for 1.23.1.3 extracted from

Tamura, M.; Tsuji, Y.; Kusunose, T.; Okazawa, A.; Kamimura, N.; Mori, T.; Nakabayashi, R.; Hishiyama, S.; Fukuhara, Y.; Hara, H.; Sato-Izawa, K.; Muranaka, T.; Saito, K.; Katayama, Y.; Fukuda, M.; Masai, E.; Kajita, S.
Successful expression of a novel bacterial gene for pinoresinol reductase and its effect on lignan biosynthesis in transgenic Arabidopsis thaliana (2014), Appl. Microbiol. Biotechnol., 98, 8165-8177 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene pinZ, recombinant expression in Arabidopsis thaliana under the control of the cauliflower mosaic virus 35S promoter, pinZ expression causes dynamic metabolic changes in stems, but not in roots and leaves. Accumulation of the glucoside of secoisolariciresinol appears to be elevated in the transgenic plant. Expression of pinZ influenced the metabolisms of lignan and glucosinolates but not so much of neolignans such as guaiacylglycerol-8-O-4'-feruloyl ethers, recombinant enzyme tissue expression pattern in plant seedlings	Sphingobium sp. SYK-6

Cloned (Comment)

Organism

gene pinZ, recombinant expression in Arabidopsis thaliana under the control of the cauliflower mosaic virus 35S promoter, pinZ expression causes dynamic metabolic changes in stems, but not in roots and leaves. Accumulation of the glucoside of secoisolariciresinol appears to be elevated in the transgenic plant. Expression of pinZ influenced the metabolisms of lignan and glucosinolates but not so much of neolignans such as guaiacylglycerol-8-O-4'-feruloyl ethers, recombinant enzyme tissue expression pattern in plant seedlings

Sphingobium sp. SYK-6

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(-)-pinoresinol + NADPH + H+	Sphingobium sp. SYK-6	-	(-)-lariciresinol + NADP+	-	?
additional information	Sphingobium sp. SYK-6	(-/+)-pinoresinol is successively converted into (-/+)-lariciresinol and (-/+)-secoisolariciresinol by enzyme PinZ	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Sphingobium sp. SYK-6	G2IMF2	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(-)-pinoresinol + NADPH + H+	-	Sphingobium sp. SYK-6	(-)-lariciresinol + NADP+	-	?
additional information	(-/+)-pinoresinol is successively converted into (-/+)-lariciresinol and (-/+)-secoisolariciresinol by enzyme PinZ	Sphingobium sp. SYK-6	?	-	?

Synonyms

Synonyms	Comment	Organism
More	cf. EC 1.23.1.4, EC 1.23.1.1, and EC 1.23.1.2	Sphingobium sp. SYK-6
pinoresinol reductase	-	Sphingobium sp. SYK-6
PinZ	-	Sphingobium sp. SYK-6

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Sphingobium sp. SYK-6

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Sphingobium sp. SYK-6

General Information

General Information	Comment	Organism
physiological function	recombinant expression of gene pinZ in Arabidopsis thaliana causes dynamic metabolic changes in stems, but not in roots and leaves. Expression of pinZ influenced the metabolisms of lignan and glucosinolates but not so much of neolignans such as guaiacylglycerol-8-O-4'-feruloyl ethers	Sphingobium sp. SYK-6