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Literature summary for 1.3.1.10 extracted from

  • Wang, P.; Denoya, C.D.; Morgenstern, M.R.; Skinner, D.D.; Kimberlee, K.W.; Digate, R.; Patton, S.; Banavali, N.; Schuler, G.; Speedie, M.K.; Reynolds, K.A.
    Cloning and characterization of the gene encoding 1-cyclohexenylcarbonyl coenzyme A reductase from Streptomyces collinus (1996), J. Bacteriol., 178, 6873-6881.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.10

Cloned(Commentary)

Cloned (Comment) Organism
chcA in pUC18 and pET3C Streptomyces collinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 trans-3,4-dihydroxycyclohexa-1,5-dienecarbonyl-CoA only impure solution available, KM and vmax in same order of magnitude as with 1-cyclohexenylcarbonyl-CoA Streptomyces collinus
0.025
-
 1-cyclohexenylcarbonyl-CoA vmax 0.0075 mmol/mg/min Streptomyces collinus
0.03
-
 5-hydroxycyclohex-1-enecarbonyl-CoA vmax 0.0053 mmol/mg/min Streptomyces collinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29700
-
 ChcA, gene sequence Streptomyces collinus
37000
-
 ChcA, SDS-PAGE, +/-2000 Streptomyces collinus
75000
-
 ChcA, gel filtration on superose 12, +/-10000 Streptomyces collinus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-cyclohexenylcarbonyl-CoA + NADPH Streptomyces collinus reaction in ansatrienin biosynthesis out of shikimic acid 1-cyclohexylcarbonyl-CoA + NADP+
-
 ?
5-hydroxycyclohex-1-enecarbonyl-CoA + NADPH Streptomyces collinus reaction in ansatrienin biosynthesis out of shikimic acid 5-hydroxycyclohexylcarbonyl-CoA + NADP+
-
 ?
trans-3,4-dihydroxycyclohexa-1,5-dienecarbonyl-CoA + NADPH Streptomyces collinus reaction in ansatrienin biosynthesis out of shikimic acid trans-4,5-dihydroxycyclohexa-2-enecarbonyl-CoA + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces collinus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
chromatography of protein overexpressed in Escherichia coli by using Sephadex G-100, DEAE, Mono-Q HR 5/5 Streptomyces collinus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.882
-
 crude Escherichia coli cell extract after overexpression Streptomyces collinus
3.667
-
 purified, overexpressed enzyme Streptomyces collinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-cyclohexenylcarbonyl-CoA + NADPH reaction in ansatrienin biosynthesis out of shikimic acid Streptomyces collinus 1-cyclohexylcarbonyl-CoA + NADP+
-
 ?
5-hydroxycyclohex-1-enecarbonyl-CoA + NADPH reaction in ansatrienin biosynthesis out of shikimic acid Streptomyces collinus 5-hydroxycyclohexylcarbonyl-CoA + NADP+
-
 ?
trans-3,4-dihydroxycyclohexa-1,5-dienecarbonyl-CoA + NADPH reaction in ansatrienin biosynthesis out of shikimic acid Streptomyces collinus trans-4,5-dihydroxycyclohexa-2-enecarbonyl-CoA + NADP+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 37000, homodimer, comparison molecular weight SDS-PAGE versus gel filtration Streptomyces collinus

Synonyms

Synonyms Comment Organism
1-cyclohexenylcarbonyl coenzyme A reductase
-
 Streptomyces collinus