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Literature summary for 1.3.1.102 extracted from

  • Matsushima, A.; Sato, Y.; Otsuka, M.; Watanabe, T.; Yamamoto, H.; Hirata, T.
    An enone reductase from Nicotiana tabacum: cDNA cloning, expression in Escherichia coli, and reduction of enones with the recombinant proteins (2008), Bioorg. Chem., 36, 23-28.
    View publication on PubMed
Search for more literature for 1.3.1.102

Cloned(Commentary)

Cloned (Comment) Organism
NtRed-1 cloned in Escherichia coli Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
 (R)-pulegone recombinant NtRed-1, pH 7.0 and 35°C Nicotiana tabacum
8.3
-
 (S)-pulegone recombinant NtRed-1, pH 7.0 and 35°C Nicotiana tabacum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36300
-
 native NtRed-1, SDS-PAGE Nicotiana tabacum
38000
-
 recombinant NtRed-1, 2 * 38000, gel filtration chromatography Nicotiana tabacum
38480
-
 recombinant NtRed-1, MALDI-TOF MS spectra Nicotiana tabacum
74000
-
 recombinant NtRed-1, gel filtration chromatography Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum Q9SLN8
-
-

Purification (Commentary)

Purification (Comment) Organism
by DEAE–Toyopearl and Red–Toyopearl column chromatography Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-pulegone + NADPH + H+
-
 Nicotiana tabacum (1R,4R)-isomenthone + (1R,4S)-menthone + NADP+
-
 ?
(S)-pulegone + NADPH + H+
-
 Nicotiana tabacum (1S,4S)-isomenthone + (1S,4R)-menthone + NADP+
-
 ?
additional information recombinant NtRed-1 does not catalyze dehydrogenation of (2S,4S)-carveol, indicating that the recombinant NtRed-1 lacks the catalytic function as allyl-alcohol dehydrogenase, although the recombinant NtRed-1 is designed from the nucleotide sequence of allyl-ADH Nicotiana tabacum ?
-
 ?

Subunits

Subunits Comment Organism
homodimer recombinant NtRed-1, 2 * 38000, gel filtration chromatography Nicotiana tabacum

Synonyms

Synonyms Comment Organism
allyl-alcohol dehydrogenase
-
 Nicotiana tabacum
NtRed-1
-
 Nicotiana tabacum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8
-
 (S)-pulegone recombinant NtRed-1, pH 7.0 and 35°C Nicotiana tabacum
3.3
-
 (R)-pulegone recombinant NtRed-1, pH 7.0 and 35°C Nicotiana tabacum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.34
-
 (S)-pulegone recombinant NtRed-1, pH 7.0 and 35°C Nicotiana tabacum
2.4
-
 (R)-pulegone recombinant NtRed-1, pH 7.0 and 35°C Nicotiana tabacum