Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is useful for the production of (S)-2-chloropropionate, which is used for the industrial production of aryloxyphenoxypropionic acid herbicides | Burkholderia sp. |
Cloned (Comment) | Organism |
---|---|
overproduced in Escherichia coli | Burkholderia sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-bromoacrylate | above 2.5 mM | Burkholderia sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
NADPH | pH 8.0, 35°C | Burkholderia sp. | |
0.19 | - |
2-bromoacrylate | pH 8.0, 35°C | Burkholderia sp. | |
0.45 | - |
2-chloroacrylate | pH 8.0, 35°C | Burkholderia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration | Burkholderia sp. |
35656 | - |
1 * 35656, calculated from sequence | Burkholderia sp. |
35660 | - |
mass spectrometric analysis | Burkholderia sp. |
37000 | - |
1 * 37000, SDS-PAGE | Burkholderia sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-bromoacrylate + NADPH + H+ | Burkholderia sp. | no activity with NADH | (S)-2-bromopropanoate + NADP+ | - |
? | |
2-chloroacrylate + NADPH + H+ | Burkholderia sp. | the enzyme is involved in the degradation of unsaturated organohalogen compounds | (S)-2-chloropropanoate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia sp. | Q59I44 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Burkholderia sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.9 | - |
2-chloroacrylate, pH 8.0, 35°C | Burkholderia sp. |
2.8 | - |
2-bromoacrylate, pH 8.0, 35°C | Burkholderia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-bromoacrylate + NADPH + H+ | no activity with NADH | Burkholderia sp. | (S)-2-bromopropanoate + NADP+ | - |
? | |
2-chloroacrylate + NADPH + H+ | no activity with NADH | Burkholderia sp. | (S)-2-chloropropanoate + NADP+ | - |
? | |
2-chloroacrylate + NADPH + H+ | the enzyme is involved in the degradation of unsaturated organohalogen compounds | Burkholderia sp. | (S)-2-chloropropanoate + NADP+ | - |
? | |
additional information | no activity with 1,4-benzoquinone, 1,4-naphthoquinone, acrylate and methacrylate | Burkholderia sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37000, SDS-PAGE | Burkholderia sp. |
monomer | 1 * 35656, calculated from sequence | Burkholderia sp. |
Synonyms | Comment | Organism |
---|---|---|
CAA43 | - |
Burkholderia sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Burkholderia sp. |
40 | - |
- |
Burkholderia sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
stable below, 30 min | Burkholderia sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Burkholderia sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | 30°C, 30 min | Burkholderia sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | no activity with NADH | Burkholderia sp. |