Application | Comment | Organism |
---|---|---|
analysis | development of a simple thermal shift assay, which does not use ACP-linked substrates, to determine the binding ability of triclosan to the enzyme's active site | Staphylococcus aureus |
Cloned (Comment) | Organism |
---|---|
- |
Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
A95V | about 15% of wild-type activtiy, no inhibition by triclosan and no shift in melting temperature in presence of both triclosan and NADP+ | Staphylococcus aureus |
F204S | no shift in melting temperature in presence of both triclosan and NADP+ | Staphylococcus aureus |
I193S | no shift in melting temperature in presence of both triclosan and NADP+ | Staphylococcus aureus |
M99T | mutant shows a similar shift in melting temperature in presence of both triclosan and NADP+ as wild-type | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
triclosan | 1.12 microM, 9% residual activity | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | A0A0H3K7V7 | - |
- |
Staphylococcus aureus Newman | A0A0H3K7V7 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.66 | - |
pH not specified in the publication, 25°C | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-[acyl-carrier-protein] + NADPH + H+ | - |
Staphylococcus aureus | butyryl-[acyl-carrier-protein] + NADP+ | - |
? | |
crotonyl-[acyl-carrier-protein] + NADPH + H+ | - |
Staphylococcus aureus Newman | butyryl-[acyl-carrier-protein] + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30397, calculated, x * 30397, mass spectrometry | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
FabI | - |
Staphylococcus aureus |
NWMN_0881 | - |
Staphylococcus aureus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
39.4 | - |
mutant A95V, melting temperature | Staphylococcus aureus |
40.2 | - |
mutant M99T, melting temperature | Staphylococcus aureus |
40.5 | - |
wild-type, melting temperature | Staphylococcus aureus |
58.8 | - |
wild-type, melting temperature in presence of both triclosan and NADP+. Neither triclosan nor NADP+ alone increases the Tm value | Staphylococcus aureus |
60.2 | - |
mutant M99T, melting temperature in presence of both triclosan and NADP+ | Staphylococcus aureus |