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Literature summary for 1.3.1.117 extracted from

  • Ibdah, M.; Berim, A.; Martens, S.; Valderrama, A.L.; Palmieri, L.; Lewinsohn, E.; Gang, D.R.
    Identification and cloning of an NADPH-dependent hydroxycinnamoyl-CoA double bond reductase involved in dihydrochalcone formation in Malus x domestica Borkh (2014), Phytochemistry, 107, 24-31 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Malus domestica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.093
-
trans-feruloyl-CoA pH 50, 35°C Malus domestica
0.097
-
trans-4-coumaroyl-CoA pH 50, 35°C Malus domestica
0.101
-
NADPH pH 50, 35°C Malus domestica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trans-4-coumaroyl-CoA + NADPH + H+ Malus domestica enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1 dihydro-4-coumaroyl-CoA + NADP+
-
?
trans-feruloyl-CoA + NADPH + H+ Malus domestica enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1 dihydroferuloyl-CoA + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Malus domestica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trans-4-coumaroyl-CoA + NADPH + H+
-
Malus domestica dihydro-4-coumaroyl-CoA + NADP+
-
?
trans-4-coumaroyl-CoA + NADPH + H+ enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1 Malus domestica dihydro-4-coumaroyl-CoA + NADP+
-
?
trans-feruloyl-CoA + NADPH + H+
-
Malus domestica dihydroferuloyl-CoA + NADP+
-
?
trans-feruloyl-CoA + NADPH + H+ enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1 Malus domestica dihydroferuloyl-CoA + NADP+
-
?

Subunits

Subunits Comment Organism
? x * 38400, SDS-PAGE and calculated from sequence Malus domestica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Malus domestica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0019
-
trans-4-coumaroyl-CoA pH 50, 35°C Malus domestica
0.0023
-
NADPH pH 50, 35°C Malus domestica
0.0042
-
trans-feruloyl-CoA pH 50, 35°C Malus domestica

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Malus domestica
NADPH
-
Malus domestica

pI Value

Organism Comment pI Value Maximum pI Value
Malus domestica calculated from sequence
-
6.9