Literature summary for 1.3.1.12 extracted from

Sun, W.; Singh, S.; Zhang, R.; Turnbull, J.L.; Christendat, D.
Crystal structure of prephenate dehydrogenase from Aquifex aeolicus. Insights into the catalytic mechanism (2006), J. Biol. Chem., 281, 12919-12928.

Cloned(Commentary)

Cloned (Comment)	Organism
into vector pET15b and expressed in Escherichia coli BL21-Gold (DE3)	Aquifex aeolicus

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with NAD+, by hanging drop vapor diffusion technique at room temperature, one modified nucleotide-binding domain and a novel helical prephenate binding domain, active site formed at the domain interface and shared between the subunits of the dimer, access to active site may be regulated via a gated mechanism, modulated by an ionic network involving a conserved arginine, active site residues include Ser126 and Lys246 and the catalytic His147, two functional domains of chorismate mutase-prephenate dehydrogenase are interdependent	Aquifex aeolicus

Crystallization (Comment)

Organism

in complex with NAD+, by hanging drop vapor diffusion technique at room temperature, one modified nucleotide-binding domain and a novel helical prephenate binding domain, active site formed at the domain interface and shared between the subunits of the dimer, access to active site may be regulated via a gated mechanism, modulated by an ionic network involving a conserved arginine, active site residues include Ser126 and Lys246 and the catalytic His147, two functional domains of chorismate mutase-prephenate dehydrogenase are interdependent

Aquifex aeolicus

Protein Variants

Protein Variants	Comment	Organism
additional information	delta19-PDH, delta25-PDH, and delta28-PDH, which produce stable and soluble proteins, delta36-PDH, delta52-PDH, and delta55-PDH are unstable	Aquifex aeolicus

Organism

Organism	UniProt	Comment	Textmining
Aquifex aeolicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by Ni-NTA column	Aquifex aeolicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
prephenate + NAD+	-	Aquifex aeolicus	4-hydroxyphenylpyruvate + NADH + CO2	-	?

Subunits

Subunits	Comment	Organism
dimer	gel filtration	Aquifex aeolicus

Synonyms

Synonyms	Comment	Organism
PDH	-	Aquifex aeolicus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Aquifex aeolicus