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Literature summary for 1.3.1.12 extracted from

  • Bonvin, J.; Aponte, R.A.; Marcantonio, M.; Singh, S.; Christendat, D.; Turnbull, J.L.
    Biochemical characterization of prephenate dehydrogenase from the hyperthermophilic bacterium Aquifex aeolicus (2006), Protein Sci., 15, 1417-1432.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.12

Activating Compound

Activating Compound Comment Organism Structure
Guanidine-HCl activates at low concentrations, at higher concentrations concomitant loss of activity with a multi-state pathway of denaturation Aquifex aeolicus

Cloned(Commentary)

Cloned (Comment) Organism
into vector pET-15b, expression as His-tagged protein in Escherichia coli Aquifex aeolicus

Protein Variants

Protein Variants Comment Organism
DELTA1-19 delta19PD, more sensitive to temperature effects yielding a half-life of 55 min at 95°C versus 2 h for PD Aquifex aeolicus

Inhibitors

Inhibitors Comment Organism Structure
Co2+ weakly inhibits Aquifex aeolicus
additional information EDTA, MgCl2 and ZnCl2 show no effect on enzyme activity Aquifex aeolicus
tyrosine feedback-inhibitor of activity over a wide temperature range, enhances the cooperativity between subunits in the binding of prephenate Aquifex aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 prephenate at 30°C, wild-type Aquifex aeolicus
0.017
-
 L-arogenate at 30°C, wild-type Aquifex aeolicus
0.025
-
 NAD+ at 30°C, wild-type Aquifex aeolicus
0.041
-
 prephenate at 30°C, delta19PD Aquifex aeolicus
0.041
-
 L-arogenate at 30°C, delta19PD Aquifex aeolicus
0.053
-
 NAD+ at 30°C, delta19PD Aquifex aeolicus
0.071
-
 NAD+ at 55°C, delta19PD Aquifex aeolicus
0.072
-
 NAD+ at 55°C, wild-type Aquifex aeolicus
0.084
-
 NAD+ at 80°C, delta19PD Aquifex aeolicus
0.097
-
 prephenate at 55°C, wild-type Aquifex aeolicus
0.097
-
 L-arogenate at 55°C, wild-type Aquifex aeolicus
0.109
-
 NAD+ at 80°C, wild-type Aquifex aeolicus
0.135
-
 prephenate at 55°C, delta19PD Aquifex aeolicus
0.135
-
 L-arogenate at 55°C, delta19PD Aquifex aeolicus
0.136
-
 prephenate at 80°C, wild-type Aquifex aeolicus
0.136
-
 L-arogenate at 80°C, wild-type Aquifex aeolicus
0.249
-
 prephenate at 80°C, delta19PD Aquifex aeolicus
0.249
-
 L-arogenate at 80°C, delta19PD Aquifex aeolicus

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl
-
 Aquifex aeolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 1 * 37000 + 1 * 34000, SDS-PAGE Aquifex aeolicus
37000
-
 1 * 37000 + 1 * 34000, SDS-PAGE Aquifex aeolicus

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by nickel-affinity chromatography Aquifex aeolicus

Storage Stability

Storage Stability Organism
-86°C Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arogenate + NAD+
-
 Aquifex aeolicus L-tyrosine + NADH + CO2
-
 ?
prephenate + NAD+
-
 Aquifex aeolicus 4-hydroxyphenylpyruvate + CO2 + NADH
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 37000 + 1 * 34000, SDS-PAGE Aquifex aeolicus

Synonyms

Synonyms Comment Organism
PD
-
 Aquifex aeolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
 Aquifex aeolicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 95
-
 Aquifex aeolicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
 NAD+ at 30°C, wild-type Aquifex aeolicus
1.3
-
 prephenate at 30°C, wild-type Aquifex aeolicus
1.3
-
 L-arogenate at 30°C, wild-type Aquifex aeolicus
1.5
-
 NAD+ at 30°C, delta19PD Aquifex aeolicus
2.2
-
 prephenate at 30°C, delta19PD Aquifex aeolicus
2.2
-
 L-arogenate at 30°C, delta19PD Aquifex aeolicus
3 6 prephenate at 80°C, wild-type Aquifex aeolicus
3 6 L-arogenate at 80°C, wild-type Aquifex aeolicus
6.7
-
 NAD+ at 55°C, wild-type Aquifex aeolicus
6.8
-
 prephenate at 55°C, wild-type Aquifex aeolicus
6.8
-
 L-arogenate at 55°C, wild-type Aquifex aeolicus
11.5
-
 NAD+ at 55°C, delta19PD Aquifex aeolicus
13
-
 prephenate at 55°C, delta19PD Aquifex aeolicus
13
-
 L-arogenate at 55°C, delta19PD Aquifex aeolicus
34
-
 NAD+ at 80°C, wild-type Aquifex aeolicus
62
-
 NAD+ at 80°C, delta19PD Aquifex aeolicus
68
-
 prephenate at 80°C, delta19PD Aquifex aeolicus
68
-
 L-arogenate at 80°C, delta19PD Aquifex aeolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 at 55°C Aquifex aeolicus