Crystallization (Comment) | Organism |
---|---|
prephenate dehydrogenase bound with NAD+ plus either 4-hydroxyphenylpyuvate, 4-hydroxyphenylpropionate, or L-tyrosine. Resiudes His147 and Arg250 are key catalytic and binding groups, respectively, and Ser126 participates in both catalysis and substrate binding through the ligand 4-hydroxyl group. Inhibitor tyrosine binds directly to the active site of the enzyme and not to an allosteric site | Aquifex aeolicus |
Protein Variants | Comment | Organism |
---|---|---|
H147N | inactive, binds prephenate with apparent affinity similar to wild-type | Aquifex aeolicus |
H217A | 40fold increase in Km for prephenate, no inhibition by tyrosine | Aquifex aeolicus |
H217N | 30fold increase in Km for prephenate, no inhibition by tyrosine | Aquifex aeolicus |
R250Q | 10fold increase in the Km for prephenate and a 20fold increase in Ki for tyrosine | Aquifex aeolicus |
S126A | 15fold reduction in kcat, a 10fold increase in the Km value for prephenate, and a 2-fold increase in Ki for tyrosine | Aquifex aeolicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tyrosine | binds directly to the active site of the enzyme and not to an allosteric site. Linear competitive inhibition | Aquifex aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NAD+ | mutant H217A, pH 7.5, 55°C | Aquifex aeolicus | |
0.012 | - |
NAD+ | mutant H217N, pH 7.5, 55°C | Aquifex aeolicus | |
0.071 | - |
NAD+ | wild-type, pH 7.5, 55°C | Aquifex aeolicus | |
0.089 | - |
NAD+ | mutant R250Q, pH 7.5, 55°C | Aquifex aeolicus | |
0.099 | - |
NAD+ | mutant S126A, pH 7.5, 55°C | Aquifex aeolicus | |
0.104 | - |
prephenate | mutant H147N, pH 7.5, 55°C | Aquifex aeolicus | |
0.135 | - |
prephenate | wild-type, pH 7.5, 55°C | Aquifex aeolicus | |
1.185 | - |
prephenate | mutant R250Q, pH 7.5, 55°C | Aquifex aeolicus | |
1.335 | - |
prephenate | mutant S126A, pH 7.5, 55°C | Aquifex aeolicus | |
3.213 | - |
prephenate | mutant H217N, pH 7.5, 55°C | Aquifex aeolicus | |
4.132 | - |
prephenate | mutant H217A, pH 7.5, 55°C | Aquifex aeolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | O67636 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate + NAD+ | - |
Aquifex aeolicus | 4-hydroxyphenylpyruvate + CO2 + NADH | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0034 | - |
prephenate | mutant H147N, pH 7.5, 55°C | Aquifex aeolicus | |
0.3 | - |
NAD+ | mutant H217N, pH 7.5, 55°C | Aquifex aeolicus | |
0.5 | - |
prephenate | mutant H217N, pH 7.5, 55°C | Aquifex aeolicus | |
0.6 | - |
NAD+ | mutant H217A, pH 7.5, 55°C | Aquifex aeolicus | |
0.8 | - |
prephenate | mutant H217A, pH 7.5, 55°C | Aquifex aeolicus | |
0.8 | - |
NAD+ | mutant S126A, pH 7.5, 55°C | Aquifex aeolicus | |
0.8 | - |
prephenate | mutant S126A, pH 7.5, 55°C | Aquifex aeolicus | |
9.9 | - |
prephenate | mutant R250Q, pH 7.5, 55°C | Aquifex aeolicus | |
11.5 | - |
NAD+ | wild-type, pH 7.5, 55°C | Aquifex aeolicus | |
11.6 | - |
NAD+ | mutant R250Q, pH 7.5, 55°C | Aquifex aeolicus | |
13 | - |
prephenate | wild-type, pH 7.5, 55°C | Aquifex aeolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Aquifex aeolicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0159 | - |
tyrosine | wild-type, pH 7.5, 55°C | Aquifex aeolicus | |
0.037 | - |
tyrosine | mutant S126A, pH 7.5, 55°C | Aquifex aeolicus | |
0.37 | - |
tyrosine | mutant R250Q, pH 7.5, 55°C | Aquifex aeolicus |