Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Saccharomyces cerevisiae |
expressed in Escherichia coli | Pleurotus ostreatus |
Protein Variants | Comment | Organism |
---|---|---|
N234D | the prephenate dehydrogenase activity of the mutant enzyme is 5fold increased compared to the wild type enzyme. L-tyrosine sensitivity is not altered compared to the wild type enzyme. The mutant does not have arognate dehydrogenase activity | Pleurotus ostreatus |
N234D | the prephenate dehydrogenase activity of the mutant enzyme is slightly reduced compared to the wild type enzyme. L-tyrosine sensitivity is not altered compared to the wild type enzyme. The mutant does not have arognate dehydrogenase activity | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-tyrosine | the enzyme is completely inhibited by 0.2 mM L-tyrosine with either NAD+ or NADP+ as cosubstrate | Pleurotus ostreatus | |
L-tyrosine | the enzyme exhibits 30% and 60% residual activity at 1 mM L-tyrosine with NAD+ or NADP+ as cosubstrate, respectively | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
prephenate | with NAD+ as cosubstrate, at pH 7.6 and 37°C | Saccharomyces cerevisiae | |
0.32 | - |
prephenate | with NAD+ as cosubstrate, at pH 7.6 and 37°C | Pleurotus ostreatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate + NAD+ | Saccharomyces cerevisiae | - |
4-hydroxyphenylpyruvate + CO2 + NADH | - |
? | |
prephenate + NAD+ | Pleurotus ostreatus | - |
4-hydroxyphenylpyruvate + CO2 + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus ostreatus | A0A386QX79 | - |
- |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
- |
Pleurotus ostreatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12000 | - |
with NADP+ as cosubstrate, pH 7.6 and 37°C | Saccharomyces cerevisiae |
30000 | - |
with NAD+ as cosubstrate, pH 7.6 and 37°C | Saccharomyces cerevisiae |
60000 | - |
with NADP+ as cosubstrate, pH 7.6 and 37°C | Pleurotus ostreatus |
300000 | - |
with NAD+ as cosubstrate, pH 7.6 and 37°C | Pleurotus ostreatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with arogenate | Saccharomyces cerevisiae | ? | - |
? | |
additional information | no activity with arogenate | Pleurotus ostreatus | ? | - |
? | |
prephenate + NAD+ | - |
Saccharomyces cerevisiae | 4-hydroxyphenylpyruvate + CO2 + NADH | - |
? | |
prephenate + NAD+ | - |
Pleurotus ostreatus | 4-hydroxyphenylpyruvate + CO2 + NADH | - |
? | |
prephenate + NADP+ | very low activity | Saccharomyces cerevisiae | 4-hydroxyphenylpyruvate + CO2 + NADPH | - |
? | |
prephenate + NADP+ | weak activity | Pleurotus ostreatus | 4-hydroxyphenylpyruvate + CO2 + NADPH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PDH | - |
Saccharomyces cerevisiae |
PDH | - |
Pleurotus ostreatus |
TYR1 | - |
Saccharomyces cerevisiae |
TYR1 | - |
Pleurotus ostreatus |
tyrA | - |
Saccharomyces cerevisiae |
tyrA | - |
Pleurotus ostreatus |
TyrA dehydrogenase | - |
Saccharomyces cerevisiae |
TyrA dehydrogenase | - |
Pleurotus ostreatus |
TyrAp | - |
Saccharomyces cerevisiae |
TyrAp | - |
Pleurotus ostreatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
prephenate | with NAD+ as cosubstrate, pH 7.6 and 37°C | Saccharomyces cerevisiae | |
3.09 | - |
prephenate | with NAD+ as cosubstrate, pH 7.6 and 37°C | Pleurotus ostreatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | preferred cofactor | Saccharomyces cerevisiae | |
NAD+ | preferred cofactor | Pleurotus ostreatus | |
NADP+ | - |
Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.62 | - |
prephenate | with NAD+ as cosubstrate, pH 7.6 and 37°C | Saccharomyces cerevisiae | |
204.33 | - |
prephenate | with NAD+ as cosubstrate, pH 7.6 and 37°C | Pleurotus ostreatus |