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Literature summary for 1.3.1.13 extracted from

  • Schenck, C.; Men, Y.; Maeda, H.
    Conserved molecular mechanism of TyrA dehydrogenase substrate specificity underlying alternative tyrosine biosynthetic pathways in plants and microbes (2017), Front. Mol. Biosci., 4, 73 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.13

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanothrix harundinacea

Protein Variants

Protein Variants Comment Organism
Q227E mutation introduces arogenate dehydrogenase activity Methanothrix harundinacea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
 prephenate wild-type, pH 7.6, temperature not specified in the publication Methanothrix harundinacea
2.67
-
 prephenate mutant Q227E, pH 7.6, temperature not specified in the publication Methanothrix harundinacea
3.29
-
 L-arogenate mutant Q227E, pH 7.6, temperature not specified in the publication Methanothrix harundinacea

Organism

Organism UniProt Comment Textmining
Methanothrix harundinacea A0A101IGG2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arogenate + NADP+ no substrate of wild-type, reaction of EC 1.3.1.78 Methanothrix harundinacea L-tyrosine + NADPH + H+ + CO2
-
 ?
prephenate + NADP+
-
 Methanothrix harundinacea 4-hydroxyphenylpyruvate + NADPH + H+ + CO2
-
 ?

Synonyms

Synonyms Comment Organism
tyrA
-
 Methanothrix harundinacea
XD72_2094
-
 Methanothrix harundinacea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.29
-
 prephenate mutant Q227E, pH 7.6, temperature not specified in the publication Methanothrix harundinacea
0.7
-
 L-arogenate mutant Q227E, pH 7.6, temperature not specified in the publication Methanothrix harundinacea
2.44
-
 prephenate wild-type, pH 7.6, temperature not specified in the publication Methanothrix harundinacea

Cofactor

Cofactor Comment Organism Structure
NAD+ enzyme strongly prefers NADP+ over NAD+ Methanothrix harundinacea
NADP+ enzyme strongly prefers NADP+ over NAD+ Methanothrix harundinacea

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.11
-
 prephenate mutant Q227E, pH 7.6, temperature not specified in the publication Methanothrix harundinacea
0.21
-
 L-arogenate mutant Q227E, pH 7.6, temperature not specified in the publication Methanothrix harundinacea
6.44
-
 prephenate wild-type, pH 7.6, temperature not specified in the publication Methanothrix harundinacea