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Literature summary for 1.3.1.14 extracted from

  • Friedmann, H.C.; Krakow, G.
    Bestimmung mit Dihydroorotat-Dehydrogenase (1984), Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. ), 2, 2010-2014.
No PubMed abstract available
Search for more literature for 1.3.1.14

Activating Compound

Activating Compound Comment Organism Structure
cysteine required for activation Faecalicatena orotica

Inhibitors

Inhibitors Comment Organism Structure
5-Methylorotate 2 mM, 50% inhibition of reduction of orotate Faecalicatena orotica
isopropyl hydantoin
-
 Faecalicatena orotica
Na3PO4 0.2 M, at pH 6.5, 20% inhibition Faecalicatena orotica
NaCl 0.2 M, 55% inhibition Faecalicatena orotica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 Orotate
-
 Faecalicatena orotica
0.13
-
 5-Fluoroorotate
-
 Faecalicatena orotica

Organism

Organism UniProt Comment Textmining
Faecalicatena orotica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Faecalicatena orotica

Storage Stability

Storage Stability Organism
above 20°C, 0.2 M phosphate buffer, fast loss of activity Faecalicatena orotica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-dihydroorotate + NAD+
-
 Faecalicatena orotica orotate + NADH + H+
-
 r
5-fluorodihydroorotate + NAD+ more active substrate for reverse reaction than orotate Faecalicatena orotica 5-fluoroorotate + NADH + H+
-
 r
additional information not: uracil, cytosine, 5-methylcytosine, thymine as substrates Faecalicatena orotica ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information values not per enzyme molecule, but per mol of flavin Faecalicatena orotica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 maximal activity Faecalicatena orotica

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein, ratio FAD to FMN 1:1 Faecalicatena orotica
flavin flavoprotein with two different flavins, probably FAD and FMN Faecalicatena orotica
FMN flavoprotein, ratio FAD to FMN 1:1 Faecalicatena orotica
additional information contains 2 mol of iron per mol of enzyme Faecalicatena orotica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.3
-
 isopropyl hydantoin
-
 Faecalicatena orotica