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Literature summary for 1.3.1.14 extracted from

  • Andersen, P.S.; Jansen, P.J.G.; Hammer, K.
    Two different dihydroorotate dehydrogenases in Lactococcus lactis (1994), J. Bacteriol., 176, 3975-3982.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain MC1061 Lactococcus lactis
two different genes Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34120
-
deduced from sequence of cDNA Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-
Lactococcus lactis A2RJT9 subspecies Lactococcus lactis cremoris, strain MG1363
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.015
-
native wild type enzyme, using NAD+ as acceptor, at 30┬░C Lactococcus lactis
0.05
-
recombinant wild type enzyme, using NAD+ as acceptor, at 37┬░C Lactococcus lactis
0.06
-
native wild type enzyme, using 2,6-dichlorophenolindophenol as acceptor, at 30┬░C Lactococcus lactis
5.9
-
recombinant wild type enzyme, using 2,6-dichlorophenolindophenol as acceptor, at 37┬░C Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-dihydroorotate + 2,6-dichlorophenolindophenol
-
Lactococcus lactis orotate + reduced 2,6-dichlorophenolindophenol
-
?
(S)-dihydroorotate + NAD+
-
Lactococcus lactis orotate + NADH + H+
-
?
dihydroorotate + acceptor acceptor: NAD+ for pyrDa gene product, fumarate for pyrDb gene product Lactococcus lactis orotate + reduced acceptor
-
?
additional information O2, fumarate, and NADP+ do not serve as electron acceptors Lactococcus lactis ?
-
?

Synonyms

Synonyms Comment Organism
DHOdehase A
-
Lactococcus lactis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Lactococcus lactis