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Literature summary for 1.3.1.16 extracted from
- Biosynthesis of nitro-compounds. III. The enzymatic reduction of beta-nitroacrylic acid to beta-nitropropionic acid (1967), Biochemistry, 6, 2253-2260.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme is inhibited by sulfhydryl compounds, e.g. cysteine at concentrations equivalent to 3-nitroacrylic acid, inhibition probably due to the reaction of the sulfhydryl compounds with 3-nitroacrylic acid rather than an inhibition of the enzyme itself | Penicillium atrovenetum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.037 | - |
NADPH | - |
Penicillium atrovenetum |  |
| 0.16 | - |
3-Nitroacrylate | - |
Penicillium atrovenetum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-nitroacrylate + NADPH | Penicillium atrovenetum | biosynthesis of beta-nitropropionic acid | 3-nitropropanoate + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium atrovenetum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Penicillium atrovenetum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Penicillium atrovenetum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.866 | - |
- |
Penicillium atrovenetum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-nitroacrylate + NADPH | highly specific | Penicillium atrovenetum | 3-nitropropanoate + NADP+ | - |
? | |
| 3-nitroacrylate + NADPH | biosynthesis of beta-nitropropionic acid | Penicillium atrovenetum | 3-nitropropanoate + NADP+ | - |
? | |
| additional information | no substrates are acrylic acid, crotonic acid, fumaric acid, cinnamic acid, 2-hydroxy-3-nitropropionic acid, shikimic acid, aconitic acid, itaconic acid, pyruvic acid, phosphoenolpyruvate, cytochrome c | Penicillium atrovenetum | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Penicillium atrovenetum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | at pH 3 about 35% of activity maximum, at pH 7 about 60% of activity maximum | Penicillium atrovenetum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Penicillium atrovenetum | |
| NADPH | NADH cannot replace NADPH | Penicillium atrovenetum | |
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Release 2023.1 (January 2023)
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