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Literature summary for 1.3.1.19 extracted from
- The metabolism of benzene by bacteria. Purification and some properties of the enzyme cis-1,2-dihydroxycyclohexa-3,5-diene (nicotinamide adenine dinucleotide) oxidoreductase (cis-benzene glycol dehydrogenase) (1973), Biochem. J., 136, 927-934.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Pseudomonas sp. |  |
General Stability
| General Stability | Organism |
|---|---|
| dilution, 0-4°C, sharp fall in activity in the first 5-10 h | Pseudomonas sp. |
| loss of activity by ammonium sulfate precipitation and subsequent dialysis, restored by Fe2+ | Pseudomonas sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Pseudomonas sp. | |
| p-chloromercuribenzoic acid | - |
Pseudomonas sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0435 | - |
NAD+ | - |
Pseudomonas sp. | |
| 0.286 | - |
cis-1,2-dihydrobenzene-1,2-diol | cosubstrate NAD+ | Pseudomonas sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | optimum Fe2+ concentration 0.1 mM | Pseudomonas sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
4 * 110000, SDS-PAGE | Pseudomonas sp. |
| 440000 | - |
ultracentrifugation | Pseudomonas sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-1,2-dihydrobenzene-1,2-diol + NAD+ | Pseudomonas sp. | - |
catechol + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19.1 | - |
cis-1,2-dihydrobenzene-1,2-diol, NAD+ | Pseudomonas sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C or frozen at -22°C, concentrated solution, several weeks | Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-1,2-dihydrobenzene-1,2-diol + NAD+ | - |
Pseudomonas sp. | catechol + NADH | - |
? | |
| cis-1,2-dihydrobenzene-1,2-diol + NAD+ | specific for the cis-form of benzene glycol and for NAD+ as a hydrogen acceptor | Pseudomonas sp. | catechol + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 110000, SDS-PAGE | Pseudomonas sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cis-1,2-dihydrocyclohexa-3,5-diene oxidoreductase | - |
Pseudomonas sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
enzyme assay | Pseudomonas sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
with NAD+ as cofactor and Fe2+ ions, 25 mM potassium phosphate buffer | Pseudomonas sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
in 25 mM-potassium phosphate buffer | Pseudomonas sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pseudomonas sp. | |
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