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Literature summary for 1.3.1.19 extracted from

  • Allen, C.C.R.; Walker, C.E.; Sharma, N.D.; Kerley, N.A.; Boyd, D.R.; Dalton, H.
    Selectivity studies of the benzene cis-dihydrodiol dehydrogenase enzyme from Pseudomonas putida ML2 with Vicinal diol substrates (2002), Biocatal. Biotransform., 20, 257-264.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.068
-
NAD+ 30┬░C, pH 8.0 Pseudomonas putida
0.12
-
cis-1,2-dihydrobenzene-1,2-diol 30┬░C, pH 8.0 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
6 * 40000, SDS-PAGE Pseudomonas putida
220000
-
gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cis-1,2-dihydrobenzene-1,2-diol + NAD+ Pseudomonas putida
-
catechol + NADH
-
?
cis-1,2-dihydrobenzene-1,2-diol + NAD+ Pseudomonas putida ML2
-
catechol + NADH
-
?
cis-1,2-dihydrobenzene-1,2-diol + NAD+ Pseudomonas putida 8859
-
catechol + NADH
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
ML2
-
Pseudomonas putida ML2
-
ML2
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose, Superdex 200, Mono Q Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
27.5
-
-
Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-methyl-hex-4-ene-1,2-diol + NAD+ 25% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida 5-methyl-hex-1,4-diene-1,2-diol + NADH + H+
-
?
5-methyl-hex-4-ene-1,2-diol + NAD+ 25% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida ML2 5-methyl-hex-1,4-diene-1,2-diol + NADH + H+
-
?
5-methyl-hex-4-ene-1,2-diol + NAD+ 25% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida 8859 5-methyl-hex-1,4-diene-1,2-diol + NADH + H+
-
?
7-methyl-oct-6-ene-1,2-diol + NAD+ 13% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida 7-methyl-oct-1,6-diene-1,2-diol + NADH + H+
-
?
7-methyl-oct-6-ene-1,2-diol + NAD+ 13% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida ML2 7-methyl-oct-1,6-diene-1,2-diol + NADH + H+
-
?
7-methyl-oct-6-ene-1,2-diol + NAD+ 13% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida 8859 7-methyl-oct-1,6-diene-1,2-diol + NADH + H+
-
?
butane-1,2-diol + NAD+ 29% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida but-1-ene-1,2-diol + NADH
-
?
butane-1,2-diol + NAD+ 29% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida ML2 but-1-ene-1,2-diol + NADH
-
?
butane-1,2-diol + NAD+ 29% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida 8859 but-1-ene-1,2-diol + NADH
-
?
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
Pseudomonas putida catechol + NADH
-
?
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
Pseudomonas putida ML2 catechol + NADH
-
?
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
Pseudomonas putida 8859 catechol + NADH
-
?
cis-1,2-dihydrobenzene-3-bromo-(R,R)-1,2-diol + NAD+ 7% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida 3-bromocatechol + NADH
-
?
cis-1,2-dihydrobenzene-3-bromo-(R,R)-1,2-diol + NAD+ 7% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida ML2 3-bromocatechol + NADH
-
?
cis-1,2-dihydrobenzene-3-bromo-(R,R)-1,2-diol + NAD+ 7% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida 8859 3-bromocatechol + NADH
-
?
cis-1,2-dihydrobenzene-3-chloro-(R,R)-1,2-diol + NAD+ 22% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida 3-chlorocatechol + NADH
-
?
cis-1,2-dihydrobenzene-3-fluoro-(R,R)-1,2-diol + NAD+ 35% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida 3-fluorocatechol + NADH
-
?
decane-1,2-diol + NAD+ 8% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida dec-1-ene-1,2-diol + NADH
-
?
nonane-1,2-diol + NAD+ 15% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida non-1-ene-1,2-diol + NADH
-
?
pentane-1,2-diol + NAD+ 28% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida pent-1-ene-1,2-diol + NADH
-
?
propane-1,2-diol + NAD+ 36% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida prop-1-ene-1,2-diol + NADH
-
?
trimethylbutane-1,2-diol + NAD+ 21% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida trimethylbut-1-ene-1,2-diol + NADH
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 40000, SDS-PAGE Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas putida