Literature summary for 1.3.1.2 extracted from

Porter, D.J.T.; Chestnut, W.G.; Merrill, B.M.; Spector, T.
Mechanism-based inactivation of dihydropyrimidine dehydrogenase by 5-ethynyluracil (1992), J. Biol. Chem., 267, 5236-5242.

General Stability

General Stability	Organism
dithiothreitol stabilizes against inactivation via 5-alkylyluracils	Mus musculus
dithiothreitol stabilizes against inactivation via 5-alkylyluracils	Homo sapiens
dithiothreitol stabilizes against inactivation via 5-alkylyluracils	Rattus norvegicus
dithiothreitol stabilizes against inactivation via 5-alkylyluracils	Bos taurus

Inhibitors

Inhibitors	Comment	Organism
5-Bromouracil	-	Bos taurus
5-Bromouracil	-	Homo sapiens
5-Bromouracil	-	Mus musculus
5-Bromouracil	-	Rattus norvegicus
5-bromvinyluracil	-	Bos taurus
5-bromvinyluracil	-	Homo sapiens
5-bromvinyluracil	-	Mus musculus
5-bromvinyluracil	-	Rattus norvegicus
5-cyanouracil	-	Bos taurus
5-cyanouracil	-	Homo sapiens
5-cyanouracil	-	Mus musculus
5-cyanouracil	-	Rattus norvegicus
5-Diazouracil	-	Bos taurus
5-Diazouracil	-	Homo sapiens
5-Diazouracil	-	Mus musculus
5-Diazouracil	-	Rattus norvegicus
5-Ethynyluracil	inactivation rate is dependent on dithiothreitol content	Bos taurus
5-Ethynyluracil	inactivation rate is dependent on dithiothreitol content	Homo sapiens
5-Ethynyluracil	inactivation rate is dependent on dithiothreitol content	Mus musculus
5-Ethynyluracil	inactivation rate is dependent on dithiothreitol content	Rattus norvegicus
5-propynyluracil	-	Bos taurus
5-propynyluracil	-	Homo sapiens
5-propynyluracil	-	Mus musculus
5-propynyluracil	-	Rattus norvegicus
5-trifluoromethyluracil	-	Bos taurus
5-trifluoromethyluracil	-	Homo sapiens
5-trifluoromethyluracil	-	Mus musculus
5-trifluoromethyluracil	-	Rattus norvegicus
additional information	no inhibition or inactivation with 5-nitro-, 5-fluoro-,5-carbamido-, 5-formyl-, 5-acetamido-, 5-hydroxymethyl- or 5-chlorouracil	Bos taurus
additional information	no inhibition or inactivation with 5-nitro-, 5-fluoro-,5-carbamido-, 5-formyl-, 5-acetamido-, 5-hydroxymethyl- or 5-chlorouracil	Homo sapiens
additional information	no inhibition or inactivation with 5-nitro-, 5-fluoro-,5-carbamido-, 5-formyl-, 5-acetamido-, 5-hydroxymethyl- or 5-chlorouracil	Mus musculus
additional information	no inhibition or inactivation with 5-nitro-, 5-fluoro-,5-carbamido-, 5-formyl-, 5-acetamido-, 5-hydroxymethyl- or 5-chlorouracil	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-
Homo sapiens	-	-	-
Mus musculus	-	-	-
Rattus norvegicus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
dihydrothymine + NADP+	-	Mus musculus	thymine + NADPH	-	r
dihydrothymine + NADP+	-	Homo sapiens	thymine + NADPH	-	r
dihydrothymine + NADP+	-	Rattus norvegicus	thymine + NADPH	-	r
dihydrothymine + NADP+	-	Bos taurus	thymine + NADPH	-	r
thymine + NADPH	-	Mus musculus	dihydrothymine + NADP+	-	?
thymine + NADPH	-	Homo sapiens	dihydrothymine + NADP+	-	?
thymine + NADPH	-	Rattus norvegicus	dihydrothymine + NADP+	-	?
thymine + NADPH	-	Bos taurus	dihydrothymine + NADP+	-	?

Cofactor

Cofactor	Comment	Organism
NADPH	-	Mus musculus
NADPH	-	Homo sapiens
NADPH	-	Rattus norvegicus
NADPH	-	Bos taurus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
additional information	-	additional information	for 5-ethynyl-, 5-diazo-, 5-cyano-, 5-bromvinyl-, 5-propynyl-, 5-bromo- and 5-trifluoromethyluracil	Mus musculus
additional information	-	additional information	for 5-ethynyl-, 5-diazo-, 5-cyano-, 5-bromvinyl-, 5-propynyl-, 5-bromo- and 5-trifluoromethyluracil	Homo sapiens
additional information	-	additional information	for 5-ethynyl-, 5-diazo-, 5-cyano-, 5-bromvinyl-, 5-propynyl-, 5-bromo- and 5-trifluoromethyluracil	Rattus norvegicus
additional information	-	additional information	for 5-ethynyl-, 5-diazo-, 5-cyano-, 5-bromvinyl-, 5-propynyl-, 5-bromo- and 5-trifluoromethyluracil	Bos taurus