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Literature summary for 1.3.1.2 extracted from
- Purification and characterization of dihydropyrimidine dehydrogenase from pig liver (1989), Eur. J. Biochem., 185, 219-224.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | rate limiting enzyme in catabolic degradation of pyrimidine derivatives, selective inhibition is strategy for design of antitumor, antimicrobial and potentially antiparasitic agents | Sus scrofa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Sus scrofa | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | iron atoms are probably present in iron-sulfur centers | Sus scrofa |  |
| Iron | contains 32 iron atoms per 206000 MW protein | Sus scrofa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 107000 | - |
2 * 107000, SDS-PAGE | Sus scrofa |
| 206000 | - |
gel filtration | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.35 | - |
- |
Sus scrofa |
| 21 | - |
purified enzyme | Sus scrofa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, protein concentration 0.5-1 mg/ml, 1 mM dithiothreitol, stable for several months | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-aminouracil + NADPH | - |
Sus scrofa | ? | - |
? | |
| uracil + NADPH | - |
Sus scrofa | 5,6-dihydrouracil + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 107000, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Sus scrofa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | contains 2 mol FMN and 2 mol FAD per mol of enzyme, tightly associated | Sus scrofa | |
| NADPH | - |
Sus scrofa | |
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