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Literature summary for 1.3.1.20 extracted from

  • Kume, T.; Iwasa, H.; Shiraishi, H.; Yokoi, T.; Nagashima, K.; Otsuka, M.; Terada, T.; Takagi, T.; Hara, A.; Kamataki, T.
    Characterization of a novel variant (S145C/L311V) of 3alpha-hydroxysteroid/dihydrodiol dehydrogenase in human liver (1999), Pharmacogenetics, 9, 763-771.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Sulfobromophthalein DD4 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
DD4 double mutant S145C/L311V overexpressed in Escherichia coli Homo sapiens
DD4, isolation of mRNA of native mutants isolated from a liver sample, synthesis of cDNA by RT-PCR, polymorphism of DD4 gene Homo sapiens

Protein Variants

Protein Variants Comment Organism
L311V native mutant with C to G replacement in DNA at position 931 Homo sapiens
S145C native mutant with C to G replacement in DNA at position 434, no significant effect on activity Homo sapiens
S145C/L311V constructed double mutant with 3 to 5fold decreased activity for xenobiotic and steroidal substrates Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Betamethasone DD4 wild-type and mutants Homo sapiens
dexamethasone DD4 wild-type and mutants Homo sapiens
Hexestrol DD4 wild-type and mutants Homo sapiens
indomethacin DD4 wild-type and mutants Homo sapiens
medroxyprogesterone DD4 wild-type and mutants Homo sapiens
additional information DD4 is inhibited by steroidal anti-inflammatory drugs Homo sapiens
Phenolphthalein DD4 wild-type and mutants Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
DD4 wild-type and mutant enzymes, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens involved in the metabolism of steroid hormones and polycyclic aromatic hydrocarbons, and also in the reduction of ketone-containing drugs ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
DD4
-

Purification (Commentary)

Purification (Comment) Organism
DD4 double mutant S145C/L311V recombinant from Escherichia coli Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ DD4 is identical with human chlordecone reductase and 3alpha-hydroxysteroid dehydrogenase type 1, EC 1.1.1.213, catalyzes the oxidoreduction of various 3alpha-hydroxysteroids including bile acids and belong to the aldo-keto reductase superfamily, in this concern termed AKR1C4 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.73
-
DD4 recombinant double mutant S145C/L311V, purified, substrate (S)-indan-1-ol Homo sapiens
1.5
-
DD4 recombinant mutant S145C, purified, substrate (S)-indan-1-ol Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-indan-1-ol + NADP+ DD4, recombinant double mutant S145C/L311V Homo sapiens ?
-
?
(R)-tetralol + NADP+ DD4, recombinant double mutant S145C/L311V Homo sapiens ?
-
?
(S)-indan-1-ol + NADP+ DD4, wild-type and recombinant double mutant S145C/L311V Homo sapiens ?
-
?
(S)-tetralol + NADP+ DD4, recombinant double mutant S145C/L311V Homo sapiens ?
-
?
3alpha-hydroxy steroid + NADP+
-
Homo sapiens 3-keto steroid + NADPH
-
?
5beta-androstan-3alpha,17beta-diol + NADP+ DD4 wild-type and mutants Homo sapiens 5beta-androstan-3,17-dione + NADPH
-
?
androsterone + NADPH DD4 wild-type and mutants Homo sapiens ?
-
?
cholic acid + NADP+ DD4, wild-type and recombinant double mutant S145C/L311V Homo sapiens ?
-
?
additional information involved in the metabolism of steroid hormones and polycyclic aromatic hydrocarbons, and also in the reduction of ketone-containing drugs Homo sapiens ?
-
?
additional information DD4 also catalyzes oxidoreduction of 3alpha-hydroxysteroids and bile acids and is thus thought to be identical with 3alpha-hydroxysteroid dehydrogenase EC 1.1.1.213 Homo sapiens additional information
-
?
pyridine-3-aldehyde + NADPH DD4 wild-type and mutants Homo sapiens ?
-
?
trans-1,2-dihydrobenzene-1,2-diol + NAD(P)+
-
Homo sapiens catechol + NAD(P)H
-
?
trans-naphthalene dihydrodiol + NAD(P)+
-
Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 36000, DD4 wild-type and mutants, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
DD
-
Homo sapiens
dihydrodiol dehydrogenase
-
Homo sapiens
More DD4 shows only 83% sequence homology to the other human DD isoforms Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at, recombinant doule mutant Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00667
-
trans-benzene dihydrodiol double mutant 145C/L311V Homo sapiens
0.0117
-
trans-benzene dihydrodiol
-
Homo sapiens
0.045
-
5beta-Androstan-3alpha,17beta-diol double mutant S145C/L311V Homo sapiens
0.0567
-
(S)-indan-1-ol double mutant S145C/L311V Homo sapiens
0.0883
-
cholic acid double mutant S145C/L311V Homo sapiens
0.108
-
5beta-Androstan-3alpha,17beta-diol
-
Homo sapiens
0.123
-
(S)-indan-1-ol
-
Homo sapiens
0.233
-
cholic acid
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Homo sapiens