Protein Variants | Comment | Organism |
---|---|---|
H132A | the mutant exhibits an increased Km value for NADPH compared to the wild type enzyme | Homo sapiens |
R124A | the mutant exhibits an increased Km value for NADPH compared to the wild type enzyme | Homo sapiens |
R174A | the mutant exhibits an increased Km value for NADPH compared to the wild type enzyme | Homo sapiens |
R35A | the mutant exhibits a strongly increased Km value for NADPH compared to the wild type enzyme | Homo sapiens |
R35S | the mutant exhibits a strongly increased Km value for NADPH compared to the wild type enzyme | Homo sapiens |
R39A | the mutant exhibits a reduced Km value for NADPH compared to the wild type enzyme | Homo sapiens |
R78A | the mutant exhibits an increased Km value for NADPH compared to the wild type enzyme | Homo sapiens |
S111A | the mutant shows strongly reduced activity compared to the wild type enzyme | Homo sapiens |
S111L | the mutant shows strongly reduced activity compared to the wild type enzyme | Homo sapiens |
W116A | the mutant exhibits a reduced Km value for NADPH compared to the wild type enzyme | Homo sapiens |
W116F | the mutant exhibits a reduced Km value for NADPH compared to the wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
NADPH | mutant enzyme W116F, at pH 7.2 and 30°C | Homo sapiens | |
0.0016 | - |
NADPH | mutant enzyme R39A, at pH 7.2 and 30°C | Homo sapiens | |
0.0017 | - |
NADPH | mutant enzyme W116A, at pH 7.2 and 30°C | Homo sapiens | |
0.0024 | - |
NADPH | wild type enzyme, at pH 7.2 and 30°C | Homo sapiens | |
0.003 | - |
NADPH | mutant enzyme R174A, at pH 7.2 and 30°C | Homo sapiens | |
0.0045 | - |
NADPH | mutant enzyme R124A, at pH 7.2 and 30°C | Homo sapiens | |
0.0047 | - |
NADPH | mutant enzyme R78A, at pH 7.2 and 30°C | Homo sapiens | |
0.0049 | - |
NADPH | mutant enzyme H132A, at pH 7.2 and 30°C | Homo sapiens | |
0.0058 | - |
NADPH | mutant enzyme S111L, at pH 7.2 and 25°C | Homo sapiens | |
0.0067 | - |
NADPH | mutant enzyme S111A, at pH 7.2 and 25°C | Homo sapiens | |
0.0083 | - |
NADPH | wild type enzyme, at pH 7.2 and 25°C | Homo sapiens | |
0.029 | - |
NADPH | mutant enzyme R35S, at pH 7.2 and 30°C | Homo sapiens | |
0.059 | - |
NADPH | mutant enzyme R35A, at pH 7.2 and 30°C | Homo sapiens | |
0.107 | - |
NADH | mutant enzyme R35S, at pH 7.2 and 30°C | Homo sapiens | |
0.108 | - |
NADH | mutant enzyme R35A, at pH 7.2 and 30°C | Homo sapiens | |
0.118 | - |
NADH | wild type enzyme, at pH 7.2 and 30°C | Homo sapiens | |
0.15 | - |
NADH | mutant enzyme R78A, at pH 7.2 and 30°C | Homo sapiens | |
0.186 | - |
NADH | mutant enzyme W116A, at pH 7.2 and 30°C | Homo sapiens | |
0.305 | - |
NADH | mutant enzyme R39A, at pH 7.2 and 30°C | Homo sapiens | |
0.319 | - |
NADH | mutant enzyme W116F, at pH 7.2 and 30°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
biliverdin IXbeta + NADH + H+ | Homo sapiens | - |
bilirubin + NAD+ | - |
r | |
biliverdin IXbeta + NADPH + H+ | Homo sapiens | - |
bilirubin + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P30043 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biliverdin IXbeta + NADH + H+ | - |
Homo sapiens | bilirubin + NAD+ | - |
r | |
biliverdin IXbeta + NADPH + H+ | - |
Homo sapiens | bilirubin + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
biliverdin IXbeta reductase | - |
Homo sapiens |
BLVRB | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADPH | mutant enzyme S111L, at pH 7.2 and 25°C | Homo sapiens | |
0.025 | - |
NADPH | mutant enzyme S111A, at pH 7.2 and 25°C | Homo sapiens | |
0.097 | - |
NADPH | mutant enzyme R124A, at pH 7.2 and 30°C | Homo sapiens | |
0.171 | - |
NADPH | wild type enzyme, at pH 7.2 and 25°C | Homo sapiens | |
0.237 | - |
NADPH | mutant enzyme H132A, at pH 7.2 and 30°C | Homo sapiens | |
0.237 | - |
NADPH | wild type enzyme, at pH 7.2 and 30°C | Homo sapiens | |
0.4 | - |
NADPH | mutant enzyme R174A, at pH 7.2 and 30°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
NADPH | mutant enzyme S111L, at pH 7.2 and 25°C | Homo sapiens | |
3.7 | - |
NADPH | mutant enzyme S111A, at pH 7.2 and 25°C | Homo sapiens | |
21 | - |
NADPH | wild type enzyme, at pH 7.2 and 25°C | Homo sapiens |