Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rattus norvegicus |
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
expressed in Escherichia coli C41(DE3) cells | Synechocystis sp. PCC 6803 |
Crystallization (Comment) | Organism |
---|---|
apo-, NADP+-bound and biliverdin-NADP+ complex forms of the enzyme, hanging drop vapor diffusion method, using 15% (w/v) PEG 4,000, 50 mM Tris-HCl (pH 7.25), 0.2 M sodium acetate and 0.2 mM Cymal-2 | Homo sapiens |
apo-, NADP+-bound and biliverdin-NADP+ complex forms of the enzyme, hanging drop vapor diffusion method, using 15% (w/v) PEG 4,000, 50mM Tris-HCl (pH 7.25), 0.2M sodium acetate and 0.2mM Cymal-2 | Rattus norvegicus |
apo-, NADP+-bound and biliverdin-NADP+ complex forms of the enzyme, hanging drop vapor diffusion method, using 15% (w/v) PEG 4,000, 50mM Tris-HCl (pH 7.25), 0.2M sodium acetate and 0.2mM Cymal-2 | Synechocystis sp. PCC 6803 |
Protein Variants | Comment | Organism |
---|---|---|
K237A | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Synechocystis sp. PCC 6803 |
R171A | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Rattus norvegicus |
R171E | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Rattus norvegicus |
R171K | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Rattus norvegicus |
R172A | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
R172E | inactive | Homo sapiens |
R172K | inactive | Homo sapiens |
R185A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Synechocystis sp. PCC 6803 |
R185K | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Synechocystis sp. PCC 6803 |
R188A | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Synechocystis sp. PCC 6803 |
R246A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Synechocystis sp. PCC 6803 |
S184A | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Synechocystis sp. PCC 6803 |
T169A | the mutant shows wild type catalytic efficiency | Synechocystis sp. PCC 6803 |
Y102F | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Synechocystis sp. PCC 6803 |
Y97F | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Rattus norvegicus |
Y98F | the mutant shows about wild type catalytic efficiency | Rattus norvegicus |
Y98F | the mutant shows about wild type catalytic efficiency | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
Biliverdin | wild type enzyme, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
0.0019 | - |
Biliverdin | mutant enzyme S184A, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
0.0033 | - |
Biliverdin | mutant enzyme R188A, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
0.0034 | - |
Biliverdin | mutant enzyme T169A, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
0.0038 | - |
Biliverdin | mutant enzyme Y98F, at pH 8.9, temperature not specified in the publication | Homo sapiens | |
0.004 | - |
Biliverdin | mutant enzyme Y102F, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
0.0046 | - |
Biliverdin | wild type enzyme, at pH 8.9, temperature not specified in the publication | Rattus norvegicus | |
0.0048 | - |
Biliverdin | mutant enzyme K237A, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
0.0051 | - |
Biliverdin | mutant enzyme R246A, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
0.0069 | - |
Biliverdin | mutant enzyme R185A, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
0.0117 | - |
Biliverdin | mutant enzyme Y97F, at pH 8.9, temperature not specified in the publication | Rattus norvegicus | |
0.0126 | - |
Biliverdin | mutant enzyme R185K, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
0.0207 | - |
Biliverdin | wild type enzyme, at pH 8.9, temperature not specified in the publication | Homo sapiens | |
0.0244 | - |
Biliverdin | mutant enzyme R171A, at pH 8.9, temperature not specified in the publication | Rattus norvegicus | |
0.0457 | - |
Biliverdin | mutant enzyme R171E, at pH 8.9, temperature not specified in the publication | Rattus norvegicus | |
0.0877 | - |
Biliverdin | mutant enzyme R172A, at pH 8.9, temperature not specified in the publication | Homo sapiens | |
0.1633 | - |
Biliverdin | mutant enzyme R171K, at pH 8.9, temperature not specified in the publication | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
biliverdin + NADH + H+ | Rattus norvegicus | - |
bilirubin + NAD+ | - |
r | |
biliverdin + NADH + H+ | Homo sapiens | - |
bilirubin + NAD+ | - |
r | |
biliverdin + NADH + H+ | Synechocystis sp. PCC 6803 | - |
bilirubin + NAD+ | - |
r | |
biliverdin + NADPH + H+ | Rattus norvegicus | - |
bilirubin + NADP+ | - |
r | |
biliverdin + NADPH + H+ | Homo sapiens | - |
bilirubin + NADP+ | - |
r | |
biliverdin + NADPH + H+ | Synechocystis sp. PCC 6803 | - |
bilirubin + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P53004 | - |
- |
Rattus norvegicus | P06762 | - |
- |
Synechocystis sp. PCC 6803 | P72782 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity gel chromatography and Sephacryl S-200 gel filtration | Rattus norvegicus |
nickel affinity gel chromatography and Sephacryl S-200 gel filtration | Homo sapiens |
nickel affinity gel chromatography and Sephacryl S-200 gel filtration | Synechocystis sp. PCC 6803 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biliverdin + NADH + H+ | - |
Rattus norvegicus | bilirubin + NAD+ | - |
r | |
biliverdin + NADH + H+ | - |
Homo sapiens | bilirubin + NAD+ | - |
r | |
biliverdin + NADH + H+ | - |
Synechocystis sp. PCC 6803 | bilirubin + NAD+ | - |
r | |
biliverdin + NADPH + H+ | - |
Rattus norvegicus | bilirubin + NADP+ | - |
r | |
biliverdin + NADPH + H+ | - |
Homo sapiens | bilirubin + NADP+ | - |
r | |
biliverdin + NADPH + H+ | - |
Synechocystis sp. PCC 6803 | bilirubin + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
BVR | - |
Rattus norvegicus |
BVR | - |
Homo sapiens |
BVR | - |
Synechocystis sp. PCC 6803 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
Biliverdin | mutant enzyme R171E, at pH 8.9, temperature not specified in the publication | Rattus norvegicus | |
0.7 | - |
Biliverdin | mutant enzyme R185A, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
0.9 | - |
Biliverdin | mutant enzyme R185K, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
2 | 3.7 | Biliverdin | mutant enzyme K237A, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
2.7 | - |
Biliverdin | mutant enzyme Y102F, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
6 | - |
Biliverdin | mutant enzyme R171A, at pH 8.9, temperature not specified in the publication | Rattus norvegicus | |
7.1 | - |
Biliverdin | wild type enzyme, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
11 | - |
Biliverdin | mutant enzyme R246A, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
11.3 | - |
Biliverdin | mutant enzyme S184A, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
13.1 | - |
Biliverdin | mutant enzyme T169A, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
13.4 | - |
Biliverdin | mutant enzyme R188A, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
13.6 | - |
Biliverdin | mutant enzyme Y97F, at pH 8.9, temperature not specified in the publication | Rattus norvegicus | |
14.7 | - |
Biliverdin | mutant enzyme R171K, at pH 8.9, temperature not specified in the publication | Rattus norvegicus | |
16.8 | - |
Biliverdin | mutant enzyme Y98F, at pH 8.9, temperature not specified in the publication | Homo sapiens | |
17.9 | - |
Biliverdin | mutant enzyme R172A, at pH 8.9, temperature not specified in the publication | Homo sapiens | |
69.3 | - |
Biliverdin | wild type enzyme, at pH 8.9, temperature not specified in the publication | Rattus norvegicus | |
94.8 | - |
Biliverdin | wild type enzyme, at pH 8.9, temperature not specified in the publication | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Rattus norvegicus | |
NADH | - |
Homo sapiens | |
NADH | - |
Synechocystis sp. PCC 6803 | |
NADPH | - |
Rattus norvegicus | |
NADPH | - |
Homo sapiens | |
NADPH | - |
Synechocystis sp. PCC 6803 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
Biliverdin | mutant enzyme R171E, at pH 8.9, temperature not specified in the publication | Rattus norvegicus | |
70 | - |
Biliverdin | mutant enzyme R185K, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
100 | - |
Biliverdin | mutant enzyme R171K, at pH 8.9, temperature not specified in the publication | Rattus norvegicus | |
100 | - |
Biliverdin | mutant enzyme R185A, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
200 | - |
Biliverdin | mutant enzyme R171A, at pH 8.9, temperature not specified in the publication | Rattus norvegicus | |
200 | - |
Biliverdin | mutant enzyme R172A, at pH 8.9, temperature not specified in the publication | Homo sapiens | |
700 | - |
Biliverdin | mutant enzyme Y102F, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
1200 | - |
Biliverdin | mutant enzyme Y97F, at pH 8.9, temperature not specified in the publication | Rattus norvegicus | |
2200 | - |
Biliverdin | mutant enzyme R246A, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
3900 | - |
Biliverdin | mutant enzyme T169A, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
3900 | - |
Biliverdin | wild type enzyme, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
4100 | - |
Biliverdin | mutant enzyme R188A, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
4400 | - |
Biliverdin | mutant enzyme Y98F, at pH 8.9, temperature not specified in the publication | Homo sapiens | |
4600 | - |
Biliverdin | wild type enzyme, at pH 8.9, temperature not specified in the publication | Homo sapiens | |
4900 | - |
Biliverdin | mutant enzyme K237A, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
5900 | - |
Biliverdin | mutant enzyme S184A, at pH 5.8, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
15100 | - |
Biliverdin | wild type enzyme, at pH 8.9, temperature not specified in the publication | Rattus norvegicus |