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Literature summary for 1.3.1.25 extracted from
- Metabolism of aromatic compounds in bacteria. Purification and properties of the catechol-forming enzyme, 3,5-cyclohexadiene-1,2-diol-1-carboxylic acid (NAD +) oxidoreductase (decarboxylating) (1972), J. Biol. Chem., 247, 4960-4965.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | - |
Cupriavidus necator |  |
| MgSO4 | - |
Cupriavidus necator | |
| Na2SO4 | depending on pH | Cupriavidus necator | |
| NaNO2 | - |
Cupriavidus necator | |
| NaNO3 | - |
Cupriavidus necator | |
| NH4Cl | - |
Cupriavidus necator | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | dependency on pH | Cupriavidus necator | |
| 0.15 | - |
NAD+ | pH 8.0 | Cupriavidus necator | |
| 0.2 | - |
(-)-3,5-cyclohexadien-1,2-diol-1-carboxylate | - |
Cupriavidus necator | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
? * 24000, SDS-PAGE | Cupriavidus necator |
| 94600 | - |
sedimentation equilibrium centrifugation | Cupriavidus necator |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ | Cupriavidus necator | - |
catechol + CO2 + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus necator | - |
basonym Alcaligenes eutrophus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cupriavidus necator |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ = catechol + CO2 + NADH + H+ | mechanism | Cupriavidus necator |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 150 | - |
purified enzyme | Cupriavidus necator |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ | - |
Cupriavidus necator | catechol + CO2 + NADH | - |
? | |
| additional information | no substrates: benzoic acid, cis-3,5-cyclohexadien-1,2-diol | Cupriavidus necator | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | ? * 24000, SDS-PAGE | Cupriavidus necator |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Cupriavidus necator |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Cupriavidus necator |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | specific for | Cupriavidus necator | |
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