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Literature summary for 1.3.1.25 extracted from

  • Reineke, W.; Knackmuss, H.J.
    Hybrid pathway for chlorobenzoate metabolism in Pseudomonas sp. B13 derivatives (1980), J. Bacteriol., 142, 467-473.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
addition of recombinant enzyme on plasmid TOL plasmid of donor PaW1 to native one in B13 derivative WR1 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ Pseudomonas sp.
-
catechol + CO2 + NADH
-
?
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ Pseudomonas sp. B13
-
catechol + CO2 + NADH
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. B13
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ = catechol + CO2 + NADH + H+ by growth on 3-chloro-, 4-chloro-, and 3,5-dichlorobenzoate enzyme is induced when combinated with recombinant toluate-1,2-dioxygenase Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
-
Pseudomonas sp. catechol + CO2 + NADH
-
?
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
-
Pseudomonas sp. B13 catechol + CO2 + NADH
-
?
3-methyldihydrodihydroxybenzoate + NAD+ assay substrate Pseudomonas sp. 3-methylcatechol + CO2 + NADH
-
?
3-methyldihydrodihydroxybenzoate + NAD+ assay substrate Pseudomonas sp. B13 3-methylcatechol + CO2 + NADH
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
native enzyme: 10 min, 90% activity, recombinant enzyme: 5 min, complete inactivation Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas sp.