Cloned (Comment) | Organism |
---|---|
addition of recombinant enzyme on plasmid TOL plasmid of donor PaW1 to native one in B13 derivative WR1 | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ | Pseudomonas sp. | - |
catechol + CO2 + NADH | - |
? | |
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ | Pseudomonas sp. B13 | - |
catechol + CO2 + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. B13 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ = catechol + CO2 + NADH + H+ | by growth on 3-chloro-, 4-chloro-, and 3,5-dichlorobenzoate enzyme is induced when combinated with recombinant toluate-1,2-dioxygenase | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ | - |
Pseudomonas sp. | catechol + CO2 + NADH | - |
? | |
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ | - |
Pseudomonas sp. B13 | catechol + CO2 + NADH | - |
? | |
3-methyldihydrodihydroxybenzoate + NAD+ | assay substrate | Pseudomonas sp. | 3-methylcatechol + CO2 + NADH | - |
? | |
3-methyldihydrodihydroxybenzoate + NAD+ | assay substrate | Pseudomonas sp. B13 | 3-methylcatechol + CO2 + NADH | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
native enzyme: 10 min, 90% activity, recombinant enzyme: 5 min, complete inactivation | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas sp. |