General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | 16.7 mM, 55% inhibition | Pseudomonas putida | |
8-hydroxyquinoline | 16.7 mM, 25% inhibition | Pseudomonas putida | |
EDTA | 16.7 mM, 17% inhibition | Pseudomonas putida | |
p-chloromercuribenzoate | 16.7 mM, 87% inhibition | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
NAD+ | - |
Pseudomonas putida | |
0.8 | - |
cis-1,2-dihydronaphthalene-1,2-diol | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25500 | - |
4 * 25500, SDS-PAGE | Pseudomonas putida |
102000 | - |
gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
strains NP, PL6 and C84-15 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas putida |
8.43 | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
4°C, 11 days, little loss of activity | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+ | i.e. cis-naphthalene dihydrodiol, specific for (+)-isomers | Pseudomonas putida | 1,2-dihydroxynaphthalene + NADH | - |
? | |
cis-1,2-dihydronaphthalene-1,2-diol + NAD+ | - |
Pseudomonas putida | naphthalene-1,2-diol + NADH + H+ | - |
r | |
cis-anthracene dihydrodiol + NAD+ | 56% of activity with cis-naphthalene dihydrodiol | Pseudomonas putida | 1,2-dihydroxyanthracene + NADH | - |
? | |
cis-benzene dihydrodiol + NAD+ | 4% of activity with cis-naphthalene dihydrodiol | Pseudomonas putida | 1,2-dihydroxybenzene + NADH | - |
? | |
cis-biphenyl dihydrodiol + NAD+ | 43% of activity with cis-naphthalene dihydrodiol | Pseudomonas putida | 3-phenyl-1,2-dihydroxybenzene + NADH | - |
? | |
cis-ethylbenzene dihydrodiol + NAD+ | 48% of activity with cis-naphthalene dihydrodiol | Pseudomonas putida | 3-ethyl-1,2-dihydroxybenzene + NADH | - |
? | |
cis-phenanthrene dihydrodiol + NAD+ | 53% of activity with cis-naphtalene dihydrodiol | Pseudomonas putida | 3,4-dihydroxyphenanthrene + NADH | - |
? | |
cis-toluene dihydrodiol + NAD+ | 26% of activity with cis-naphthalene dihydrodiol | Pseudomonas putida | 3-methyl-1,2-dihydroxybenzene + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 25500, SDS-PAGE | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | 9 | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | absolutely required | Pseudomonas putida |