General Stability | Organism |
---|---|
highly labile in low buffer concentration | Rattus norvegicus |
markedly stabilized in 20% glycerol | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00219 | - |
progesterone | - |
Rattus norvegicus | |
0.0024 | - |
testosterone | - |
Rattus norvegicus | |
0.003 | - |
NADPH | - |
Rattus norvegicus | |
0.00485 | - |
4-androstene-3,17-dione | - |
Rattus norvegicus | |
0.0091 | - |
7alpha-hydroxy-4-cholesten-3-one | - |
Rattus norvegicus | |
0.011 | - |
cortisol | - |
Rattus norvegicus | |
0.0122 | - |
7alpha,12alpha-dihydroxy-4-cholesten-3-one | - |
Rattus norvegicus | |
0.0217 | - |
cortisone | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
SDS-PAGE, gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-androstene-3,17-dione + NADPH | Rattus norvegicus | - |
5beta-androstan-3,17-dione + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cortisone 5beta-reduction (EC 1.3.1.3) and androstenedione reduction (EC 1.3.1.23) are catalyzed by the same catalytic site of a single protein | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01528 | - |
- |
Rattus norvegicus |
0.0153 | - |
purified enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-androstene-3,17-dione + NADPH | - |
Rattus norvegicus | 5beta-androstan-3,17-dione + NADP+ | - |
? | |
7alpha,12alpha-dihydroxy-4-cholesten-3-one + NADPH | - |
Rattus norvegicus | 7alpha,12alpha-dihydroxy-5-beta-cholestan-3-one + NADP+ | - |
? | |
7alpha-hydroxy-4-cholesten + NADPH | - |
Rattus norvegicus | 7alpha-hydroxy-5beta-cholestan-3-one + NADP+ | - |
? | |
7alpha-hydroxy-4-cholesten-3-one + NADPH + H+ | - |
Rattus norvegicus | 7alpha-hydroxy-5beta-cholestan-3-one + NADP+ | - |
r | |
cortisol + NADPH | - |
Rattus norvegicus | ? | - |
? | |
cortisone + NADPH | - |
Rattus norvegicus | ? | - |
? | |
additional information | NADH: no effect | Rattus norvegicus | ? | - |
? | |
progesterone + NADPH | - |
Rattus norvegicus | ? | - |
? | |
testosterone + NADPH + H+ | - |
Rattus norvegicus | (5beta,17beta)-17-hydroxyandrostan-3-one + NADP+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00817 | - |
cortisol | - |
Rattus norvegicus | |
0.01 | - |
7alpha-hydroxy-4-cholesten-3-one | - |
Rattus norvegicus | |
0.0128 | - |
7alpha,12alpha-dihydroxy-4-cholesten-3-one | - |
Rattus norvegicus | |
0.0208 | - |
testosterone | - |
Rattus norvegicus | |
0.0302 | - |
progesterone | - |
Rattus norvegicus | |
0.0337 | - |
4-androstene-3,17-dione | - |
Rattus norvegicus | |
0.0375 | - |
cortisone | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
7alpha-hydroxy-4-cholesten-3-one | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 8.2 | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | NADH: no effect | Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |