BRENDA - Enzyme Database
show all sequences of 1.3.1.3

Molecular cloning and expression of progesterone 5beta-reductase (5beta-POR) from Isoplexis canariensis

Herl, V.; Fischer, G.; Bötsch, R.; Müller-Uri, F.; Kreis, W.; Planta Med. 72, 1163-1165 (2006)

Data extracted from this reference:

Application
Application
Commentary
Organism
additional information
significant homology to the putative progesterone 5beta-reductases isolated from other plant species, such as Digitalis lanata (ca. 98% identical) and Arabidopsis thaliana (ca. 69% identical)
Digitalis canariensis
Cloned(Commentary)
Cloned (Commentary)
Organism
SPHI /SALI IC5beta-POR fragment cloned into the pQE vector system and transformed into Escherichai coli strain M15(pREP4)
Digitalis canariensis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.015
-
NADPH
-
Digitalis canariensis
0.215
-
progesterone
-
Digitalis canariensis
2.079
-
cortisol
-
Digitalis canariensis
2.193
-
4-androstene-3,17-dione
-
Digitalis canariensis
2.373
-
cortexone
-
Digitalis canariensis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
x * 45000, SDS–PAGE
Digitalis canariensis
Organism
Organism
UniProt
Commentary
Textmining
Digitalis canariensis
Q078S6
-
-
Purification (Commentary)
Purification (Commentary)
Organism
by Ni-NTA affinity chromatography
Digitalis canariensis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Digitalis canariensis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-androstene-3,17-dione + NADPH
-
676735
Digitalis canariensis
5beta-androstan-3,15-dione + NADP+
-
-
-
?
cortexone + NADPH
-
676735
Digitalis canariensis
21-trihydroxy-5beta-pregnane-3,20-dione + NADP+
-
-
-
?
cortisol + NADPH
-
676735
Digitalis canariensis
11beta,17,21-trihydroxy-5beta-pregnane-3,20-dione + NADP+
-
-
-
?
progesterone + NADPH + H+
-
676735
Digitalis canariensis
5beta-pregnane-3,20-dione + NADP+
-
-
-
ir
Subunits
Subunits
Commentary
Organism
?
x * 45000, SDS–PAGE
Digitalis canariensis
Synonyms
Synonyms
Commentary
Organism
5beta-POR
-
Digitalis canariensis
EC 1.3.1.3
related
Digitalis canariensis
progesterone 5beta-reductase
-
Digitalis canariensis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Digitalis canariensis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Digitalis canariensis
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Digitalis canariensis
Application (protein specific)
Application
Commentary
Organism
additional information
significant homology to the putative progesterone 5beta-reductases isolated from other plant species, such as Digitalis lanata (ca. 98% identical) and Arabidopsis thaliana (ca. 69% identical)
Digitalis canariensis
Cloned(Commentary) (protein specific)
Commentary
Organism
SPHI /SALI IC5beta-POR fragment cloned into the pQE vector system and transformed into Escherichai coli strain M15(pREP4)
Digitalis canariensis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Digitalis canariensis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.015
-
NADPH
-
Digitalis canariensis
0.215
-
progesterone
-
Digitalis canariensis
2.079
-
cortisol
-
Digitalis canariensis
2.193
-
4-androstene-3,17-dione
-
Digitalis canariensis
2.373
-
cortexone
-
Digitalis canariensis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
x * 45000, SDS–PAGE
Digitalis canariensis
Purification (Commentary) (protein specific)
Commentary
Organism
by Ni-NTA affinity chromatography
Digitalis canariensis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Digitalis canariensis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-androstene-3,17-dione + NADPH
-
676735
Digitalis canariensis
5beta-androstan-3,15-dione + NADP+
-
-
-
?
cortexone + NADPH
-
676735
Digitalis canariensis
21-trihydroxy-5beta-pregnane-3,20-dione + NADP+
-
-
-
?
cortisol + NADPH
-
676735
Digitalis canariensis
11beta,17,21-trihydroxy-5beta-pregnane-3,20-dione + NADP+
-
-
-
?
progesterone + NADPH + H+
-
676735
Digitalis canariensis
5beta-pregnane-3,20-dione + NADP+
-
-
-
ir
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 45000, SDS–PAGE
Digitalis canariensis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Digitalis canariensis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Digitalis canariensis
Other publictions for EC 1.3.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726120
Bauer
Vein patterning 1-encoded prog ...
Digitalis lanata, Arabidopsis thaliana
Phytochemistry
77
53-59
2012
-
-
2
1
15
-
-
12
-
-
-
4
-
2
-
-
1
-
-
-
-
-
4
-
4
2
-
-
12
2
-
-
2
-
-
-
-
-
2
2
1
15
-
-
-
-
12
-
-
-
4
-
-
-
1
-
-
-
-
4
-
2
-
-
12
2
-
-
-
-
4
4
-
12
12
724713
Mindnich
The effect of disease associat ...
Homo sapiens
Chem. Biol. Interact.
191
250-254
2011
-
-
1
-
5
-
-
-
-
-
-
3
-
5
-
-
-
-
-
-
-
-
3
-
2
-
-
-
-
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1
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1
1
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5
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3
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3
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-
-
-
-
-
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-
3
3
-
-
-
726492
Chen
Substrate specificity and inhi ...
Homo sapiens
Steroids
76
484-490
2011
-
-
-
-
-
-
6
11
-
-
-
-
-
3
-
-
-
-
-
-
-
-
12
-
3
1
-
-
11
1
-
-
1
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-
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-
1
-
-
-
-
6
-
11
-
-
-
-
-
-
-
-
-
-
-
-
12
-
1
-
-
11
1
-
-
-
-
2
2
-
11
11
725399
Drury
Characterization of disease-re ...
Homo sapiens
J. Biol. Chem.
285
24529-24537
2010
-
-
2
-
10
-
1
9
-
-
-
4
-
5
-
-
1
-
-
-
-
-
5
1
3
1
-
-
8
1
-
-
1
2
-
-
-
-
2
1
-
10
-
-
2
2
9
-
-
-
4
-
-
-
1
-
-
-
-
5
1
1
-
-
8
1
-
-
-
-
1
1
-
8
8
725633
McNeilly
Bile acids modulate glucocorti ...
Rattus norvegicus
J. Hepatol.
52
705-711
2010
-
1
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
697264
Di Costanzo
Aldo-keto reductases in which ...
Digitalis lanata
Chem. Biol. Interact.
178
127-133
2009
-
-
-
1
-
-
-
-
-
-
-
4
-
3
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704908
Ueki
SRD5B1 gene analysis needed fo ...
Homo sapiens
J. Gastroenterol. Hepatol.
24
776-785
2009
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
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-
2
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1
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-
2
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-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
705710
Di Costanzo
Structure and catalytic mechan ...
Homo sapiens
Mol. Cell. Endocrinol.
301
191-198
2009
-
-
-
1
1
-
-
-
-
-
-
4
-
4
-
-
-
2
-
1
-
-
5
-
3
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685271
Faucher
The crystal structure of human ...
Homo sapiens
Biochemistry
47
8261-8270
2008
-
-
1
1
-
-
-
-
-
-
1
-
-
3
-
-
1
1
-
1
-
-
3
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687723
Di Costanzo
Crystal structure of human liv ...
Homo sapiens
J. Biol. Chem.
283
16830-16839
2008
-
-
1
1
2
-
1
1
-
-
-
-
-
3
-
-
1
-
-
2
-
-
3
-
3
-
-
-
-
-
-
-
1
1
-
-
-
-
1
1
1
2
-
-
1
1
1
-
-
-
-
-
-
-
1
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698762
Thorn
The crystal structure of proge ...
Digitalis lanata
J. Biol. Chem.
283
17260-17269
2008
-
-
1
1
2
-
-
1
-
-
-
-
-
8
-
-
1
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
2
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702225
Faucher
Crystal structures of human De ...
Homo sapiens
Biochemistry
47
13537-13546
2008
-
-
1
1
2
-
3
4
-
-
-
-
-
6
-
-
1
-
-
-
-
-
5
1
5
1
-
-
3
1
-
-
1
-
-
-
-
-
1
1
1
2
-
-
3
-
4
-
-
-
-
-
-
-
1
-
-
-
-
5
1
1
-
-
3
1
-
-
-
-
-
-
-
3
3
706774
Palermo
Human Delta4-3-oxosteroid 5bet ...
Homo sapiens
Steroids
73
417-423
2008
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
671346
Gavidia
Plant progesterone 5beta-reduc ...
Digitalis purpurea
Phytochemistry
68
853-864
2007
-
1
1
-
-
-
-
2
-
-
2
-
-
12
-
-
1
-
-
5
-
-
2
1
2
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
2
-
-
2
-
-
-
-
1
-
5
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671101
Egerer-Sieber
Crystallization and preliminar ...
Digitalis lanata
Acta Crystallogr. Sect. F
62
186-188
2006
-
1
1
1
-
-
-
-
-
-
-
-
-
7
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
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1
1
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1
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1
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-
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-
-
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-
-
-
-
-
-
-
-
676376
Herl
Molecular cloning and heterolo ...
Digitalis lanata
Phytochemistry
67
225-231
2006
-
1
1
-
-
-
-
5
-
-
1
-
-
11
-
-
1
-
-
1
-
-
6
1
2
1
-
-
-
1
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
5
-
-
1
-
-
-
-
1
-
1
-
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
676735
Herl
Molecular cloning and expressi ...
Digitalis canariensis
Planta Med.
72
1163-1165
2006
-
1
1
-
-
-
-
5
-
-
1
-
-
14
-
-
1
-
-
1
-
-
4
1
3
1
-
-
-
1
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
5
-
-
1
-
-
-
-
1
-
1
-
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
390597
Charbonneau
Genomic organization of a huma ...
Homo sapiens
Biochim. Biophys. Acta
1517
228-235
2001
-
-
2
-
-
-
-
-
-
-
-
6
-
1
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390598
Dauphin-Villemant
-
Towards the elucidation of the ...
Pancrustacea
Ann. N. Y. Acad. Sci.
839
306-310
1998
-
-
-
-
-
-
-
-
1
-
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1
-
1
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-
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1
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1
-
1
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-
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-
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-
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1
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1
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-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390650
Jez
Engineering steroid 5beta-redu ...
Rattus norvegicus
Biochemistry
37
9695-9703
1998
-
-
-
-
-
-
-
2
-
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-
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-
1
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2
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2
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2
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390590
Furuebisu
Identification of cortisone 5b ...
Rattus norvegicus
Biochim. Biophys. Acta
912
110-114
1987
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390591
Okuda
Purification and characterizat ...
Rattus norvegicus
J. Biol. Chem.
259
7519-7524
1984
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390592
Wilton
Is a Schiff base involved in t ...
Rattus norvegicus
Biochem. J.
155
487-491
1976
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390593
Collins
Subcellular localization and p ...
Mus musculus
Biochem. J.
147
165-174
1975
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390649
Ozon
Transformation of testosterone ...
Pelophylax esculentus
Biochim. Biophys. Acta
348
425-437
1974
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9
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390596
Van Doorn
Studies on partially purified ...
Sus scrofa
Can. J. Biochem.
51
1661-1668
1973
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390595
Nduaguba
Steroid DELTA4-reductases of p ...
Sus scrofa
Can. J. Biochem.
49
385-392
1971
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390646
Brown-Grant
The DELTA4-hydrogenases of gui ...
Cavia porcellus, Rattus norvegicus
J. Biol. Chem.
235
1317-1320
1960
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18
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390647
Tomkins
The enzymatic reduction of DEL ...
Bos taurus, Cavia porcellus, Columba sp., Oryctolagus cuniculus, Rattus norvegicus, Sus scrofa
J. Biol. Chem.
225
13-24
1957
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6
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390594
Forchielli
Separation of DELTA4-5alpha- a ...
Rattus norvegicus
J. Biol. Chem.
223
443-448
1956
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