Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.3 extracted from

  • Chen, M.; Wangtrakuldee, P.; Zang, T.; Duan, L.; Gathercole, L.L.; Tomlinson, J.W.; Penning, T.M.
    Human and murine steroid 5beta-reductases (AKR1D1 and AKR1D4) insights into the role of the catalytic glutamic acid (2019), Chem. Biol. Interact., 305, 163-170 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0057
-
4-androstene-3,17-dione isoform AKR1D4L, pH 7, 37°C Mus musculus
0.0071
-
cortisol isoform AKR1D4L, pH 7, 37°C Mus musculus
0.008
-
4-androstene-3,17-dione isoform AKR1D4S, pH 7, 37°C Mus musculus
0.0114
-
cortisol isoform AKR1D4S, pH 7, 37°C Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q8VCX1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
liver expression of total AKR1D4 transcripts is 100fold higher in liver versus testis Mus musculus
-
testis expression of total AKR1D4 transcripts is 100fold higher in liver versus testis Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-androstene-3,17-dione + NADPH + H+
-
Mus musculus 5beta-androstan-3,15-dione + 3alpha-hydroxy-5beta-androstan-17-one + NADP+
-
?
cortisol + NADPH + H+
-
Mus musculus 11beta,17,21-trihydroxy-5beta-pregnane-3,20-dione + NADP+
-
?
progesterone + NADPH + H+
-
Mus musculus 5beta-pregnane-3,20-dione + NADP+
-
?

Subunits

Subunits Comment Organism
More there exists a long-form, with an 18 amino-acid extension at the N-terminus (AKR1D4L) and a short-form (AKR1D4S) Mus musculus

Synonyms

Synonyms Comment Organism
AKR1D1
-
Mus musculus
AKR1D4
-
Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
4-androstene-3,17-dione isoform AKR1D4L, pH 7, 37°C Mus musculus
0.08
-
cortisol isoform AKR1D4L, pH 7, 37°C Mus musculus
0.09
-
4-androstene-3,17-dione isoform AKR1D4S, pH 7, 37°C Mus musculus
0.13
-
cortisol isoform AKR1D4S, pH 7, 37°C Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.93
-
4-androstene-3,17-dione isoform AKR1D4L, pH 7, 37°C Mus musculus
1
-
4-androstene-3,17-dione isoform AKR1D4S, pH 7, 37°C Mus musculus
10.5
-
cortisol isoform AKR1D4S, pH 7, 37°C Mus musculus
13
-
cortisol isoform AKR1D4L, pH 7, 37°C Mus musculus