Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Capsella rubella |
Crystallization (Comment) | Organism |
---|---|
homology modeling of structure | Capsella rubella |
Protein Variants | Comment | Organism |
---|---|---|
R63K | mutant is able to use NADH as cofactor | Capsella rubella |
R63K/R64H | increases enzymatic activity by 13.8fold with NADH as cofactor | Capsella rubella |
R64G | mutant exhibits significantly improved activity with NADH | Capsella rubella |
R64H | mutant exhibits significantly improved activity with NADH | Capsella rubella |
R64S | mutant exhibits significantly improved activity with NADH | Capsella rubella |
R64T | mutant exhibits significantly improved activity with NADH | Capsella rubella |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.358 | - |
2-cyclohexen-1-one | mutant R63K/R64H, cofactor NADPH, pH 6, 40°C | Capsella rubella | |
0.443 | - |
2-cyclohexen-1-one | wild-type, cofactor NADPH, pH 6, 40°C | Capsella rubella | |
0.541 | - |
2-cyclohexen-1-one | mutant R63K/R64H, cofactor NADH, pH 6, 40°C | Capsella rubella |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Capsella rubella | R0F4W3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(20S)-21-hydroxy-20-methylpregn-4-en-3-one + NADPH + H+ | - |
Capsella rubella | ? + NADP+ | - |
? | |
2-cyclohexen-1-one + NADPH + H+ | - |
Capsella rubella | ? + NADP+ | - |
? | |
4-androstene-3,17-dione + NADPH + H+ | - |
Capsella rubella | 5beta-androstan-3,17-dione + NADP+ | - |
? | |
ethyl acrylate + NADPH + H+ | - |
Capsella rubella | ? + NADP+ | - |
? | |
progesterone + NADPH + H+ | - |
Capsella rubella | 5beta-pregnane-3,20-dione + NADP+ | - |
? | |
testosterone + NADPH + H+ | - |
Capsella rubella | (5beta,17beta)-17-hydroxyandrostan-3-one + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CARUB_v10005016mg | - |
Capsella rubella |
St5betaR1 | - |
Capsella rubella |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
2-cyclohexen-1-one | wild-type, cofactor NADPH, pH 6, 40°C | Capsella rubella | |
0.28 | - |
2-cyclohexen-1-one | mutant R63K/R64H, cofactor NADPH, pH 6, 40°C | Capsella rubella | |
0.81 | - |
2-cyclohexen-1-one | mutant R63K/R64H, cofactor NADH, pH 6, 40°C | Capsella rubella |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | wild-type is strictly dependen on NADPH | Capsella rubella |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
2-cyclohexen-1-one | wild-type, cofactor NADPH, pH 6, 40°C | Capsella rubella | |
0.611 | - |
2-cyclohexen-1-one | mutant R63K/R64H, cofactor NADPH, pH 6, 40°C | Capsella rubella | |
1.5 | - |
2-cyclohexen-1-one | mutant R63K/R64H, cofactor NADH, pH 6, 40°C | Capsella rubella |