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Literature summary for 1.3.1.31 extracted from

  • Thanos, I.; Bader, J.; Guenther, H.; Neumann, S.; Krauss, F.; Simon, H.
    Electroenzymatic and electromicrobial reduction: preparation of chiral compounds (1987), Methods Enzymol., 136, 302-317.
No PubMed abstract available
Search for more literature for 1.3.1.31

General Stability

General Stability Organism
enzyme immobilized in calcium alginate gel, used in operation for 2 weeks, half-life of about 200 h Clostridium tyrobutyricum

Inhibitors

Inhibitors Comment Organism Structure
Aliphatic carboxylates
-
 Clostridium tyrobutyricum
methyl viologen
-
 Clostridium tyrobutyricum
NAD+
-
 Clostridium tyrobutyricum
phenyl propionate
-
 Clostridium tyrobutyricum
reduced methyl viologen
-
 Clostridium tyrobutyricum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 NADH
-
 Clostridium tyrobutyricum
0.013
-
 cinnamate
-
 Clostridium tyrobutyricum
0.014
-
 (E)-geraniate
-
 Clostridium tyrobutyricum
0.1
-
 (Z)-geraniate
-
 Clostridium tyrobutyricum
0.4
-
 reduced methyl viologen
-
 Clostridium tyrobutyricum
0.5
-
 (Z)-2-bromocinnamate
-
 Clostridium tyrobutyricum
1.5
-
 butenoate
-
 Clostridium tyrobutyricum

Organism

Organism UniProt Comment Textmining
Clostridium tyrobutyricum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridium tyrobutyricum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20
-
 (E)-2-methylbutenoate Clostridium tyrobutyricum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-2-methyl-2-butenoate + NADH
-
 Clostridium tyrobutyricum 2-methylbutanoate + NAD+
-
 ?
(E)-2-methylbutenoate + NADH
-
 Clostridium tyrobutyricum (R)-2-methylbutanoate + NAD+
-
 ?
(E)-2-methylbutenoate + reduced methylviologen
-
 Clostridium tyrobutyricum (R)-2-methylbutanoate + methyl viologen
-
 ?
(E)-geraniate + NADH
-
 Clostridium tyrobutyricum ?
-
 ?
(Z)-2-bromocinnamate + NADH
-
 Clostridium tyrobutyricum 3-(2-bromophenyl)propionate + NAD+
-
 ?
(Z)-geraniate + NADH
-
 Clostridium tyrobutyricum ?
-
 ?
butenoate + NADH
-
 Clostridium tyrobutyricum butanoate + NAD+
-
 ?
butenoate + reduced methyl viologen
-
 Clostridium tyrobutyricum butanoate + methyl viologen
-
 ?
cinnamate + NADH
-
 Clostridium tyrobutyricum phenylpropionate + NAD+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
 Clostridium tyrobutyricum

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6.2 about 65% of maximal activity at pH 4.5 and at pH 6.2 Clostridium tyrobutyricum

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Clostridium tyrobutyricum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.84
-
 NAD+
-
 Clostridium tyrobutyricum
10
-
 reduced methyl viologen
-
 Clostridium tyrobutyricum
20
-
 methyl viologen
-
 Clostridium tyrobutyricum
30
-
 phenyl propionate
-
 Clostridium tyrobutyricum