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Literature summary for 1.3.1.31 extracted from
- Asymmetric reduction of activated alkenes using an enoate reductase from Gluconobacter oxydans (2011), Appl. Microbiol. Biotechnol., 89, 79-89.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as a His-tagged fusion protein in Escherichia coli | Gluconobacter oxydans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
NADPH | pH 7.0, 37°C | Gluconobacter oxydans |  |
| 0.82 | - |
(E)-2-hexenal | pH 7.0, 37°C | Gluconobacter oxydans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39240 | - |
calculated from cDNA | Gluconobacter oxydans |
| 44000 | - |
SDS-PAGE | Gluconobacter oxydans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gluconobacter oxydans | - |
- |
- |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Gluconobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E)-2-hexenal + NADPH | relative activity: 100% | Gluconobacter oxydans | hexanal + NADP+ | - |
? | |
| (E)-3-nonen-2-one + NADPH | relative activity: 61.6% | Gluconobacter oxydans | nonan-2-one + NADP+ | - |
? | |
| 2,6,6-trimethyl-2-cyclohexen-1,4-dione + NADPH | relative activity: 103% | Gluconobacter oxydans | ? | - |
? | |
| 2-cyclohexen-1-one + NADPH | relative activity: 56.7% | Gluconobacter oxydans | ? | - |
? | |
| 2-methylcyclopenten-1-one + NADPH | relative activity: 12.4% | Gluconobacter oxydans | ? | - |
? | |
| citral + NADPH | relative activity: 34% | Gluconobacter oxydans | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| enoate reductase | - |
Gluconobacter oxydans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
only 25% residual activity | Gluconobacter oxydans |
| 37 | - |
assay at | Gluconobacter oxydans |
| 45 | - |
optimal activity | Gluconobacter oxydans |
| 53 | - |
only 25% residual activity | Gluconobacter oxydans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 30 | enzyme stable over 24 h | Gluconobacter oxydans |
| 59 | - |
midpoint thermal inactivation | Gluconobacter oxydans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
optimal activity in TEA buffer | Gluconobacter oxydans |
| 7 | - |
optimal activity incitrate-phosphate buffer | Gluconobacter oxydans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | required | Gluconobacter oxydans | |
| NADH | - |
Gluconobacter oxydans | |
| NADPH | NADPH is preferred, showing an 8fold higher specific activity | Gluconobacter oxydans | |
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Release 2023.1 (January 2023)
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